Infection with Cowpea severe mosaic virus (CPSMV) represents one of the main limitations for cowpea (Vigna unguiculata L. Walp.) productivity due to the severity of the disease symptoms, frequency of incidence, and difficulties in dissemination control. This study aimed to identify the proteins and metabolic pathways associated with the susceptibility and resistance of cowpea plants to CPSMV. Therefore, we treated the seeds of a naturally susceptible cowpea genotype (CE-31) with the mutagenic agent ethyl methane sulfonate (EMS) and compared the secondary leaf proteomic profile of the mutagenized resistant plants inoculated with CPSMV (MCPI plant group) to those of the naturally susceptible cowpea genotype CE-31 inoculated (CPI) and noninoculated (CPU) with CPSMV. MCPI responded to CPSMV by accumulating proteins involved in the oxidative burst, increasing H2O2 generation, promoting leaf cell death (LCD), increasing the synthesis of defense proteins, and decreasing host factors important for the establishment of CPSMV infection. In contrast, CPI accumulated several host factors that favor CPSMV infection and did not accumulate H2O2 or present LCD, which allowed CPSMV replication and systemic dissemination. Based on these results, we propose that the differential abundance of defense proteins and proteins involved in the oxidative burst, LCD, and the decrease in cowpea protein factors required for CPSMV replication are associated with the resistance trait acquired by the MCPI plant group.

中文翻译：

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H2O2 积累、宿主细胞死亡和病毒复制所需的光合作用和氧化还原稳态相关蛋白质的差异积累：解释 EMS 诱变豇豆对豇豆严重花叶病毒的抗性

豇豆严重花叶病毒 (CPSMV) 感染是豇豆 (Vigna unguiculata L. Walp.) 生产力的主要限制之一，原因是疾病症状的严重性、发病率和传播控制的困难。本研究旨在鉴定与豇豆植物对 CPSMV 的易感性和抗性相关的蛋白质和代谢途径。因此，我们用诱变剂甲磺酸乙酯 (EMS) 处理天然易感豇豆基因型 (CE-31) 的种子，并将接种 CPSMV 的诱变抗性植物（MCPI 植物组）的次生叶蛋白质组学谱与用 CPSMV 接种 (CPI) 和未接种 (CPU) 的天然易感豇豆基因型 CE-31。MCPI 通过积累参与氧化爆发的蛋白质来响应 CPSMV，增加 H2O2 生成，促进叶细胞死亡 (LCD)，增加防御蛋白的合成，并减少对 CPSMV 感染建立重要的宿主因素。相比之下，CPI 积累了几种有利于 CPSMV 感染的宿主因素，并且没有积累 H2O2 或存在 LCD，这允许 CPSMV 复制和全身传播。基于这些结果，我们提出防御蛋白和参与氧化爆发、LCD 的蛋白质的丰度差异以及 CPSMV 复制所需的豇豆蛋白因子的减少与 MCPI 植物群获得的抗性性状有关。CPI 积累了几种有利于 CPSMV 感染的宿主因素，并且没有积累 H2O2 或存在 LCD，这使得 CPSMV 复制和全身传播。基于这些结果，我们提出防御蛋白和参与氧化爆发、LCD 的蛋白质的差异丰度以及 CPSMV 复制所需的豇豆蛋白因子的减少与 MCPI 植物群获得的抗性性状有关。CPI 积累了几种有利于 CPSMV 感染的宿主因素，并且没有积累 H2O2 或存在 LCD，这使得 CPSMV 复制和全身传播。基于这些结果，我们提出防御蛋白和参与氧化爆发、LCD 的蛋白质的差异丰度以及 CPSMV 复制所需的豇豆蛋白因子的减少与 MCPI 植物群获得的抗性性状有关。