An estradiol-17β/miRNA-26a/cyp19a1a regulatory feedback loop in the protogynous hermaphroditic fish, Epinephelus coioides.,Molecular and Cellular Endocrinology

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An estradiol-17β/miRNA-26a/cyp19a1a regulatory feedback loop in the protogynous hermaphroditic fish, Epinephelus coioides.
Molecular and Cellular Endocrinology ( IF 3.8 ) Pub Date : 2019-12-28 , DOI: 10.1016/j.mce.2019.110689
Qi Yu ₁ , Cheng Peng ₂ , Zhifeng Ye ₃ , Zhujing Tang ₃ , Shuisheng Li ₄ , Ling Xiao ₄ , Su Liu ₅ , Yuqing Yang ₅ , Mi Zhao ₃ , Yong Zhang ₆ , Haoran Lin ₃

Affiliation

State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266373, China; Southern Marine Science and Engineering Guangdong Laboratory (ZhanJiang), Fisheries College, Guangdong Ocean University, Zhanjiang, 524088, China.
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, PR China; Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, 510260, China.
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, PR China.
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, PR China; Southern Marine Science and Engineering Guangdong Laboratory (ZhanJiang), Fisheries College, Guangdong Ocean University, Zhanjiang, 524088, China.
Marine Fisheries Development Center of Guangdong Province, Huizhou, 516081, China.
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266373, China; Southern Marine Science and Engineering Guangdong Laboratory (ZhanJiang), Fisheries College, Guangdong Ocean University, Zhanjiang, 524088, China; Marine Fisheries Development Center of Guangdong Province, Huizhou, 516081, China.

Cyp19a1a is a key gene responsible for the production of estradiol-17β (E2), the main functional estrogen and a major downstream regulator of reproduction in teleost fish. It is widely known that CYP19 gene expression, aromatase activity, and E2 production can influence gonadal differentiation and sex reversal in teleost fish, but the feedback mechanisms whereby E2 regulates cyp19a1a remain poorly understood, especially regarding the potential roles of endogenous small RNA molecules (miRNAs). Here, we identified miR-26a-5p as a regulatory factor of its predicted target gene (cyp19a1a). In vitro and in vivo studies showed that miR-26a-5p can decrease cyp19a1a expression. Furthermore, high doses of E2 act as a repressor of miR-26a-5p. This study proposes a regulatory feedback loop whereby E2 regulates cyp19a1a through miR-26a-5p, and suggests that this positive feedback is an important aspect of the control of E2 production.

中文翻译：

雌性雌雄同体鱼类（Epinephelus coioides）中的雌二醇-17β/ miRNA-26a / cyp19a1a调节反馈环。

Cyp19a1a是负责产生雌二醇17β（E2），主要功能雌激素和硬骨鱼繁殖的主要下游调节因子的关键基因。众所周知，CYP19基因的表达，芳香化酶活性和E2的产生可以影响硬骨鱼的性腺分化和性逆转，但是对E2调节cyp19a1a的反馈机制仍然知之甚少，特别是关于内源性小RNA分子（miRNA ）。在这里，我们确定了miR-26a-5p是其预测的目标基因（cyp19a1a）的调节因子。体外和体内研究表明，miR-26a-5p可以降低cyp19a1a的表达。此外，高剂量的E2可以作为miR-26a-5p的阻遏物。这项研究提出了一个监管反馈回路，其中E2通过miR-26a-5p调节cyp19a1a，

更新日期：2019-12-29

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