The continuous exchange of chemicals with blood and the non-invasive collection make saliva an interesting specimen for clinical applications, from the detection of biomarkers to the new –omic sciences in medicine. However, saliva sampling is challenging because the suitability of the collection method for the analyte of interest is either poorly investigated or, more often, not mentioned at all in most publications. This review reports a critical evaluation of the most common procedures for saliva sampling and a description of the off-the-shelf sampling devices. Their suitability for bioanalytical applications and salivary biomarkers detection, e.g. representativeness of the sample, sampling feasibility, analyte recovery, and sample amount, is discussed.

从生物标志物的检测到新的医学组学技术，唾液与血液之间的化学物质不断交换和无创采集使唾液成为临床应用中有趣的标本。然而，唾液采样具有挑战性，因为收集方法对目标分析物的适用性研究不充分，或者在大多数出版物中根本没有提及。该评价报告对唾液采样的最常用程序进行了严格的评估，并描述了现成的采样设备。讨论了它们对生物分析应用和唾液生物标志物检测的适用性，例如样品的代表性，采样可行性，分析物回收率和样品量。