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Preparation of a dimer from self-complementary of cobalt(III) complex with dissymmetric compartmental ligand and study of the interaction of the complex with DNA and BSA
Journal of Coordination Chemistry ( IF 2.2 ) Pub Date : 2019-11-02 , DOI: 10.1080/00958972.2019.1694148
Leila Zarei 1 , Zahra Asadi 1 , Erika Samolova 2, 3 , Michal Dusek 2
Affiliation  

Abstract Synthesis of [Co(L)(H2O)(HOCN(CH3)2)](ClO4) (where L denotes N,N′-bis(3-methoxysalicylidene)-1,2-diaminopropane) was reported. The complex was characterized by 1H NMR, elemental analysis, IR and UV–vis spectroscopy and the structural features have been solved by X-ray crystallography. The crystal structure analysis showed that the arrangement around cobalt atom is octahedral with two imine nitrogen atoms (N1 and N2) and two oxygen atoms of hydroxyl groups (O2 and O3) at equatorial positions and an aqua ligand and a DMF ligand at the axial positions. The hydrogen-bonding interactions between coordinated H2O and phenoxo/methoxy oxygens connect the complex molecules in dimers. Interaction of the complex with FS-DNA and protein (BSA) was explored by fluorescence and absorption spectroscopy, viscosity measurements, circular dichroism (CD), cyclic voltammetry (CV) and agarose gel electrophoresis. According to the Kb value about 103, the complex is moderately bound to DNA. The complex interacts with protein (BSA) by both static and dynamic quenching. According to cleavage experiment, the complex destroys annular DNA and causes double strand break. Molecular docking was done to achieve detailed binding information of the complex to DNA and BSA. Moreover, cytotoxic activity was evaluated by MTT assay. The complex has low toxicity against the MCF-7 and LNCaP cancer cells. Graphical Abstract

中文翻译:

钴(III)复合物与不对称区室配体的自互补二聚体的制备及复合物与 DNA 和 BSA 相互作用的研究

摘要 报道了 [Co(L)(H2O)(HOCN(CH3)2)](ClO4)(其中 L 表示 N,N'-双(3-甲氧基水杨烯)-1,2-二氨基丙烷)的合成。该配合物通过 1H NMR、元素分析、IR 和 UV-vis 光谱表征,结构特征已通过 X 射线晶体学解析。晶体结构分析表明,钴原子周围呈八面体排列,赤道位置有两个亚胺氮原子(N1和N2)和羟基的两个氧原子(O2和O3),轴向位置有一个水配体和一个DMF配体. 协调的 H2O 和苯氧基/甲氧基氧之间的氢键相互作用将复杂的分子连接到二聚体中。通过荧光和吸收光谱、粘度测量、圆二色性 (CD)、循环伏安法 (CV) 和琼脂糖凝胶电泳。根据 Kb 值约为 103,该复合物与 DNA 适度结合。该复合物通过静态和动态淬灭与蛋白质 (BSA) 相互作用。根据切割实验,该复合物破坏环状DNA并导致双链断裂。进行分子对接以获得复合物与 DNA 和 BSA 的详细结合信息。此外,通过MTT测定评估细胞毒活性。该复合物对 MCF-7 和 LNCaP 癌细胞具有低毒性。图形概要 该复合物破坏环状 DNA 并导致双链断裂。进行分子对接以获得复合物与 DNA 和 BSA 的详细结合信息。此外,通过MTT测定评估细胞毒活性。该复合物对 MCF-7 和 LNCaP 癌细胞具有低毒性。图形概要 该复合物破坏环状 DNA 并导致双链断裂。进行分子对接以获得复合物与 DNA 和 BSA 的详细结合信息。此外,通过MTT测定评估细胞毒活性。该复合物对 MCF-7 和 LNCaP 癌细胞具有低毒性。图形概要
更新日期:2019-11-02
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