当前位置：
X-MOL 学术
›
ChemElectroChem
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Amplified Electrochemical Aptasensor for Sialic Acid Based on Carbon‐Cloth‐Supported Gold Nanodendrites and Functionalized Gold Nanoparticles
ChemElectroChem ( IF 3.5 ) Pub Date : 2020-01-16 , DOI: 10.1002/celc.201902049 Yubing Lv 1 , Yanli Zhou 2 , Hui Dong 2 , Lantao Liu 2, 3 , Guoliang Mao 1 , Yintang Zhang 2 , Maotian Xu 2, 3
ChemElectroChem ( IF 3.5 ) Pub Date : 2020-01-16 , DOI: 10.1002/celc.201902049 Yubing Lv 1 , Yanli Zhou 2 , Hui Dong 2 , Lantao Liu 2, 3 , Guoliang Mao 1 , Yintang Zhang 2 , Maotian Xu 2, 3
Affiliation
|
The abnormal expression of sialic acid (SA) on the cell surface or in bodily fluids has been proven to correlate with several diseases. An electrochemical approach has been developed for the sensitive and accurate determination of SA in human serum samples. Gold nanodendrites (AuNDs) electrodeposited on a carbon cloth (CC) electrode were utilized as the substrate electrode and provided high surface area and conductivity. The thiolated nucleic acid aptamer of SA was immobilized and recognized SA specifically, followed by the attachment of the nanocomposite of gold nanoparticles with 4‐mercaptophenylboric acid and thionine (MPBA−AuNP−Th) via boronic acid‐diol binding. An amplified signal by the electrochemical reduction of Th could be obtained because of high conductivity of substrate and high loading of Th on AuNPs. The proposed sensor exhibited remarkable selectivity and high sensitivity with a low detection limit of 60 nM, owing to the high affinity of aptamer and the signal amplification strategy, respectively. The feasibility of the aptasensor was verified by evaluation of SA level in human serum.
中文翻译：
基于碳纤维支撑金纳米树突和功能化金纳米粒子的唾液酸放大电化学适应传感器
唾液酸（SA）在细胞表面或体液中的异常表达已被证明与几种疾病有关。已经开发出一种电化学方法来灵敏而准确地测定人血清样品中的SA。电沉积在碳布（CC）电极上的金纳米树突（AuNDs）被用作基底电极，并提供高表面积和导电性。将SA的硫醇化核酸适体固定并特异性识别SA，然后通过硼酸-二醇键合将金纳米颗粒的纳米复合物与4-巯基苯基硼酸和硫氨酸（MPBA-AuNP-Th）结合。由于底物的高电导率和Th在AuNPs上的高负载，可以通过Th的电化学还原获得放大的信号。由于适体的亲和力高和信号放大策略不同，拟议的传感器表现出显着的选择性和高灵敏度，检测限低至60 nM。适体传感器的可行性通过评估人血清中SA的含量得到了验证。
更新日期：2020-01-17
中文翻译：
基于碳纤维支撑金纳米树突和功能化金纳米粒子的唾液酸放大电化学适应传感器
唾液酸（SA）在细胞表面或体液中的异常表达已被证明与几种疾病有关。已经开发出一种电化学方法来灵敏而准确地测定人血清样品中的SA。电沉积在碳布（CC）电极上的金纳米树突（AuNDs）被用作基底电极，并提供高表面积和导电性。将SA的硫醇化核酸适体固定并特异性识别SA，然后通过硼酸-二醇键合将金纳米颗粒的纳米复合物与4-巯基苯基硼酸和硫氨酸（MPBA-AuNP-Th）结合。由于底物的高电导率和Th在AuNPs上的高负载，可以通过Th的电化学还原获得放大的信号。由于适体的亲和力高和信号放大策略不同，拟议的传感器表现出显着的选择性和高灵敏度，检测限低至60 nM。适体传感器的可行性通过评估人血清中SA的含量得到了验证。
京公网安备 11010802027423号