A series of biocompatible and non- toxic polysaccharide molecules have been successfully fabricated and explored their potential application for scavenging the carbonyl species in vitro. These macromolecules were dextrans with different hydrazide substitution ratios determined by TNBS assay, NMR and FTIR characterization. The colorimetric assay had demonstrated that these macromolecules could effectively scavenge acrolein, oxidized bovine serum albumin (BSA) in buffer solutions as well as carbonyl proteins from serum. The scavengers could achieve twice more scavenging effects for modified dextrans with high molecular weight (Mw = 100,000) than those of low ones (Mw = 40,000) with the same substitution ratio. Protein gel electrophoresis confirmed that the formation of the complex between carbonyls and modified dextrans resulted in appearance of slower bands. It also revealed that such macromolecules could protect cultured cells against the toxicity of acrolein or its derivatives. The proposed macromolecules indicated a very promising capability as scavengers for oxidative stress plus its derivatives without side effects.

中文翻译：

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氨基甲酸酯改性的葡聚糖作为羰基化蛋白的清除剂。

已经成功地制备了一系列生物相容性和无毒的多糖分子，并探索了它们在体外清除羰基物种方面的潜在应用。这些大分子是通过TNBS分析，NMR和FTIR表征确定的具有不同酰肼取代率的右旋糖酐。比色法表明，这些大分子可以有效清除缓冲液中的丙烯醛，氧化的牛血清白蛋白（BSA）以及血清中的羰基蛋白。对于具有相同分子量的低分子量（Mw = 40,000）的高分子量右旋糖酐（Mw = 100,000），具有相同取代率的清除剂的清除效果要高出两倍。蛋白凝胶电泳证实羰基和修饰的葡聚糖之间的复合物的形成导致出现较慢的条带。还揭示了这样的大分子可以保护培养的细胞免受丙烯醛或其衍生物的毒性。所提出的大分子显示出非常有前途的作为清除氧化剂及其衍生物的无毒副作用的能力。