The aim of this study was to use of bacterial cellulose/polypyrrole/TiO2-Ag (BC/PPy/TiO2-Ag) nanocomposite film to detect and measure the growth of 5 pathogenic bacteria. For this purpose, at first, 13 BC/PPy/TiO2-Ag films were fabricated, then bacterial suspensions were prepared according to McFarland standard. The results showed that by increasing the bacterial concentration, the electrical resistance of sensors was decreased and there was a relation between bacterial concentration and bacterial type with electrical resistance change of sensors. The obtained data showed that the sensitivity of the sensors was increased with increasing the concentration of polypyrrole and TiO2-Ag. FT-IR and SEM tests were performed to investigate the interaction between nanoparticles and determine the size of nanoparticles. The BC/PPy/TiO2-Ag biosensors are portable and the response time of these sensors is very short for target analysis. Therefore, these sensors have the potential to improve biological safety as diagnostic tools.

中文翻译：

---

聚吡咯/ TiO2-Ag纳米复合材料修饰的细菌纤维素膜在检测和测量病原菌生长中的用途。

这项研究的目的是利用细菌纤维素/聚吡咯/ TiO2-Ag（BC / PPy / TiO2-Ag）纳米复合膜来检测和测量5种致病细菌的生长。为此，首先要制作13层BC / PPy / TiO2-Ag膜，然后根据McFarland标准制备细菌悬液。结果表明，随着细菌浓度的增加，传感器的电阻降低，细菌浓度和细菌类型与传感器的电阻变化之间存在一定的关系。获得的数据表明，传感器的灵敏度随着聚吡咯和TiO2-Ag浓度的增加而增加。进行了FT-IR和SEM测试，以研究纳米粒子之间的相互作用并确定纳米粒子的尺寸。BC / PPy / TiO2-Ag生物传感器是便携式的，这些传感器的响应时间非常短，可用于目标分析。因此，这些传感器具有提高生物安全性作为诊断工具的潜力。