Colon carcinoma is a recurring type of cancer that affects the intestine epithelial with a poor survival rate. It was already proven the anticancer property of hesperidin in various cancers but the bioavailability hesperidin is poor, which hinders the hesperidin usage. In this investigation we synthesized hesperidin loaded Zn2+@ SA/PCT nanocomposites and assessed its anticancer potential against colon cancer (HCT116) cells. Hesperidin loaded Zn2+@ SA/PCT nanocomposites were characterized using Fourier transform infrared (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM) and transmission electron microscope (TEM) analysis. The drug releasing capacity and cytotoxic property was assessed via drug releasing assay, MTT assay with HCT116 cells. The anticancer potency of hesperidin nanocomposites were evaluated with TUNEL, DAPI staining, reactive oxygen species (ROS) generation assay and it is confirmed with flow cytometry analysis of MMP disruption in colon cancer (HCT116) cell line. Further the immunoblotting analysis of cysteine proteases Caspases 3, 9, PARP, proapoptotic protein Bax and antiapoptotic protein Bcl2 were performed. The results of FTIR, XRD and electroscopic analyses confirmed the synthesized hesperidin nanocomposites accomplish the properties of potent nanodrug and the MTT assay authentically confirmed that the synthesized hesperidin nanocomposite inhibited the HCT116 cell growth, and the results of fluorescent staining proved that the hesperidin nanocomposite induced the apoptotic mediated cell necrosis via promoting the expression of apoptotic proteins thereby induced the apoptosis in colon cancer (HCT116) cells. Hence, it was concluded that the, hesperidin loaded nanocomposites persuasively inhibited proliferation of colon carcinoma cell and induced apoptosis in in vitro condition.

中文翻译：

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橙皮苷负载的Zn2 + @ SA / PCT纳米复合材料通过增强促凋亡蛋白的表达抑制结肠癌细胞（HCT116）的增殖并诱导其凋亡。

结肠癌是影响肠上皮的复发型癌症，存活率很低。已经证实橙皮苷在各种癌症中的抗癌特性，但是橙皮苷的生物利用度差，这阻碍了橙皮苷的使用。在这项研究中，我们合成了橙皮苷负载的Zn2 + @ SA / PCT纳米复合材料，并评估了其对结肠癌（HCT116）细胞的抗癌潜力。使用傅立叶变换红外（FTIR），X射线衍射（XRD），扫描电子显微镜（SEM）和透射电子显微镜（TEM）分析对负载橙皮苷的Zn2 + @ SA / PCT纳米复合材料进行表征。通过药物释放测定，使用HCT116细胞的MTT测定来评估药物释放能力和细胞毒性性质。用TUNEL，DAPI染色评估橙皮苷纳米复合材料的抗癌能力，活性氧（ROS）生成测定，并通过流式细胞术分析结肠癌细胞（HCT116）细胞中MMP破坏得到证实。进一步进行了半胱氨酸蛋白酶Caspases 3、9，PARP，促凋亡蛋白Bax和抗凋亡蛋白Bcl2的免疫印迹分析。FTIR，XRD和电镜分析的结果证实了合成的橙皮苷纳米复合物具有强效纳米药物的性能，MTT分析证实了合成的橙皮苷纳米复合物抑制了HCT116细胞的生长，荧光染色的结果证明了橙皮苷纳米复合物诱导了HCT116细胞的生长。凋亡介导的细胞坏死通过促进凋亡蛋白的表达从而诱导结肠癌（HCT116）细胞凋亡。因此，得出的结论是，