Abstract To get new insight on the pathogenicity of Fusarium oxysporum f. sp. lycopersici, metabolic labeling was performed, and secretomes of the wild type strain 4287 (W) and non-virulent mutant ΔchsV (M) were compared while switching the main carbon source from glucose (Glc) to the freeze-dried vascular tomato tissue (VTT). 14N- or 15N-containing KNO3 was used for fungal growth and two combined (14N/15N) protein extracts were obtained: 1- W + M in Glc; 2- W + M in VTT. Trypsin digests of 1 and 2 were analyzed by capHPLC-ESI-QTOF-MS and Skyline was applied to compare the abundances of individual proteins between two strains in each sample and between the samples. Protein grouping was suggested as follows: (1) seven proteins apparently adsorbing on VTT were of importance during the initial stage of invasion; (2) three adsorbing on VTT were involved in compensative mutant adjustment, in growth and nutrients supply; (3) eleven might contribute in pathogenicity without direct interaction with the plant cell wall; (4) fourteen were involved in mutant adaptation, fungal growth and nutrients supply, not excluding their role in pathogenicity. The above proteins comprised carbohydrate-active enzymes, protein/peptide- and lipid-hydrolyzing enzymes, few classified as effectors (NEP-like, serine protease, GLEYA adhesion domain, LysM domain), catalyzing redox processes and few of unknown function.

中文翻译：

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两种尖孢镰刀菌的比较评价。sp. 在两种不同碳源上生长的番茄菌株：基于 LC-MS 的体内 15N 代谢标记后的分泌组研究

摘要 对尖孢镰刀菌的致病性有新的认识。sp. 在番茄中，进行代谢标记，比较野生型菌株 4287 (W) 和无毒突变体 ΔchsV (M) 的分泌组，同时将主要碳源从葡萄糖 (Glc) 转换为冷冻干燥的番茄维管组织 (VTT) ）。含 14N 或 15N 的 KNO3 用于真菌生长，获得两种组合 (14N/15N) 蛋白质提取物：1-W + M in Glc；2- VTT 中的 W + M。1 和 2 的胰蛋白酶消化物通过 capHPLC-ESI-QTOF-MS 进行分析，并应用 Skyline 来比较每个样品中两个菌株之间以及样品之间的单个蛋白质的丰度。蛋白质分组建议如下：（1）在入侵的初始阶段，明显吸附在VTT上的7种蛋白质是重要的；(2)三种吸附在VTT上参与补偿突变调整、生长和养分供应；(3) 11 种可能在不与植物细胞壁直接相互作用的情况下促进致病性；(4) 14 个参与突变体适应、真菌生长和营养供应，不排除它们在致病性中的作用。上述蛋白质包括碳水化合物活性酶、蛋白质/肽和脂质水解酶，少数被归类为效应物（NEP 样、丝氨酸蛋白酶、GLEYA 粘附域、LysM 域），催化氧化还原过程和少数未知功能。不排除它们在致病性中的作用。上述蛋白质包括碳水化合物活性酶、蛋白质/肽和脂质水解酶，少数被归类为效应物（NEP 样、丝氨酸蛋白酶、GLEYA 粘附域、LysM 域），催化氧化还原过程和少数未知功能。不排除它们在致病性中的作用。上述蛋白质包括碳水化合物活性酶、蛋白质/肽和脂质水解酶，少数被归类为效应物（NEP 样、丝氨酸蛋白酶、GLEYA 粘附域、LysM 域），催化氧化还原过程和少数未知功能。