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Metagenomic insights into bioaugmentation and biovalorization of oily industrial wastes by lipolytic oleaginous yeast Yarrowia lipolytica during successive batch fermentation.
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2019-12-27 , DOI: 10.1002/bab.1878 Yasmi Louhasakul 1 , Benjamas Cheirsilp 1 , Laura Treu 2 , Panagiotis G Kougias 2, 3 , Irini Angelidaki 3
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2019-12-27 , DOI: 10.1002/bab.1878 Yasmi Louhasakul 1 , Benjamas Cheirsilp 1 , Laura Treu 2 , Panagiotis G Kougias 2, 3 , Irini Angelidaki 3
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The lipolytic oleaginous yeast Yarrowia lipolytica was used in the bioaugmentation and biovalorization of oily industrial wastes during successive‐batch fermentation. Five cycles of nonsterile successive batch fermentation with 70% medium replacement achieved the highest oil removal of 68.1 ± 5.60% and produced biomass and lipid yields of 0.213 ± 0.07 g/g‐COD and 146.2 ± 46.5 mg/g‐COD, respectively. The cell‐bound lipase activity observed in the system was 170.74 ± 32 U/L. The auto‐flocculation efficiency of the biomass was >90% within 60 Min. The microbial community changes between Y. lipolytica and indigenous microorganisms were monitored by metagenomic next‐generation sequencing of internal transcribed spacer rDNA regions for yeasts and 16S rRNA gene for bacteria. Ylipolytica lipolytica was retained in the consortium together with other indigenous strains until the fifth cycle. Other minor oleaginous yeasts such as Kodamaea ohmeri and Candida tropicalis as well as polyhydroxyalkanoate‐accumulating bacteria were found and are likely to have participated in lipid production. This study has shown the robustness of Y. lipolytica in nonsterile successive batch fermentation and its use could contribute greatly to the practical valorization of industrial wastes for lipids and lipases.
中文翻译：
在连续分批发酵过程中，通过脂解性油质酵母解脂耶氏酵母（Yarrowia lipolytica）对油性工业废物进行生物强化和生物增值的元基因组学见解。
脂解性油质酵母解脂耶氏酵母用于连续分批发酵过程中油性工业废料的生物强化和生物增值。通过五个循环进行的70％培养基替代的非无菌连续分批发酵，最高除油率为68.1±5.60％，产生的生物量和脂质产量分别为0.213±0.07 g / g-COD和146.2±46.5 mg / g-COD。在系统中观察到的细胞结合脂肪酶活性为170.74±32 U / L。在60分钟内，生物质的自絮凝效率> 90％。解脂耶氏酵母与本地微生物之间的微生物群落变化通过酵母菌的内部转录间隔区rDNA区和细菌的16S rRNA基因的宏基因组下一代测序进行监测。解脂耶氏酵母与其他本地菌株一起被保留在财团中，直到第五个周期。还发现了其他较小的含油酵母，例如：柯达玛（Kodamaea ohmeri）和热带念珠菌（Candida Tropicalis），以及聚羟基链烷酸积累细菌，它们很可能参与了脂质的生产。这项研究表明解脂耶氏酵母在非无菌连续分批发酵中的稳健性，其应用可为工业废物中脂类和脂肪酶的实用增值做出很大贡献。
更新日期：2019-12-27
中文翻译：
在连续分批发酵过程中，通过脂解性油质酵母解脂耶氏酵母（Yarrowia lipolytica）对油性工业废物进行生物强化和生物增值的元基因组学见解。
脂解性油质酵母解脂耶氏酵母用于连续分批发酵过程中油性工业废料的生物强化和生物增值。通过五个循环进行的70％培养基替代的非无菌连续分批发酵，最高除油率为68.1±5.60％，产生的生物量和脂质产量分别为0.213±0.07 g / g-COD和146.2±46.5 mg / g-COD。在系统中观察到的细胞结合脂肪酶活性为170.74±32 U / L。在60分钟内，生物质的自絮凝效率> 90％。解脂耶氏酵母与本地微生物之间的微生物群落变化通过酵母菌的内部转录间隔区rDNA区和细菌的16S rRNA基因的宏基因组下一代测序进行监测。解脂耶氏酵母与其他本地菌株一起被保留在财团中，直到第五个周期。还发现了其他较小的含油酵母，例如：柯达玛（Kodamaea ohmeri）和热带念珠菌（Candida Tropicalis），以及聚羟基链烷酸积累细菌，它们很可能参与了脂质的生产。这项研究表明解脂耶氏酵母在非无菌连续分批发酵中的稳健性，其应用可为工业废物中脂类和脂肪酶的实用增值做出很大贡献。
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