Stripe rust is a fungal disease that has devastated the barley production for a long time. The present study focused on the role of β-glucan, PR proteins, diamine oxidase (DAO), polyamine oxidase (PAO), key enzymes and metabolites of phenol and proline metabolism in the stripe rust resistance of barley. RD2901 with resistant behavior against stripe rust showed increased levels of PR proteins, phenylalanine ammonia lyase (PAL), tyrosine ammonia lyase (TAL) along with the accumulation of β-glucan and lignin which strengthen the plant cell wall during plant-pathogen interaction. It also depicted the enhanced activities of glutamate dehydrogenase (GDH) and ornithine aminotransferase (OAT) coupled with the increased amounts of proline, glycine betaine and choline after infection with M-race of P. striiformis f. sp. hordei. On the contrary, the sensitive genotype Jyoti was unable to enhance the activities of most of these enzymes except PAL and OAT so that it showed an increase in lignin and choline contents only. Secondly, the increase in lignin content was less as compared to the tolerant genotype. Hence, it can be inferred that these key metabolites and enzymes of various metabolic pathways may contribute to the resistance of barley against stripe rust pathogen. This study suggested that these key enzymes and their metabolites could serve as markers for the characterization of plant defensive state that is essential for crop protection.

中文翻译：

---

大麦基因型与条锈病病原体相容相互作用期间的代谢调节。

条锈病是一种真菌病，长期以来已经破坏了大麦的产量。本研究的重点是β-葡聚糖，PR蛋白，二胺氧化酶（DAO），聚胺氧化酶（PAO），酚的关键酶和代谢产物以及脯氨酸代谢在大麦的抗条锈性中的作用。具有条纹锈病抗性的RD2901显示PR蛋白，苯丙氨酸氨裂合酶（PAL），酪氨酸氨裂合酶（TAL）的水平增加，并且β-葡聚糖和木质素的积累在植物与病原体相互作用期间增强了植物细胞壁。它也描述了在被P.striiformis f感染后，谷氨酸脱氢酶（GDH）和鸟氨酸氨基转移酶（OAT）的活性增强，加上脯氨酸，甘氨酸甜菜碱和胆碱的含量增加。sp。部落 相反，除PAL和OAT外，敏感基因型Jyoti无法增强大多数酶的活性，因此仅显示出木质素和胆碱含量的增加。其次，与耐受基因型相比，木质素含量的增加较少。因此，可以推断出这些关键的代谢物和各种代谢途径的酶可能有助于大麦对条锈病病原体的抗性。这项研究表明，这些关键酶及其代谢产物可以作为表征植物防御状态的标记，而防御状态对于作物的保护至关重要。与耐性基因型相比，木质素含量的增加较少。因此，可以推断出这些关键的代谢物和各种代谢途径的酶可能有助于大麦对条锈病病原体的抗性。这项研究表明，这些关键酶及其代谢产物可以作为表征植物防御状态的标记，而防御状态对于作物的保护至关重要。与耐性基因型相比，木质素含量的增加较少。因此，可以推断出这些关键的代谢物和各种代谢途径的酶可能有助于大麦对条锈病病原体的抗性。这项研究表明，这些关键酶及其代谢产物可以作为表征植物防御状态的标记，而防御状态对于作物的保护至关重要。