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Co-delivery of curcumin and Bcl-2 siRNA by PAMAM dendrimers for enhancement of the therapeutic efficacy in HeLa cancer cells.
Colloids and Surfaces B: Biointerfaces ( IF 5.4 ) Pub Date : 2019-12-27 , DOI: 10.1016/j.colsurfb.2019.110762 Maryam Ghaffari 1 , Gholamreza Dehghan 2 , Behzad Baradaran 3 , Amir Zarebkohan 4 , Behzad Mansoori 3 , Jafar Soleymani 5 , Jafar Ezzati Nazhad Dolatabadi 6 , Michael R Hamblin 7
Colloids and Surfaces B: Biointerfaces ( IF 5.4 ) Pub Date : 2019-12-27 , DOI: 10.1016/j.colsurfb.2019.110762 Maryam Ghaffari 1 , Gholamreza Dehghan 2 , Behzad Baradaran 3 , Amir Zarebkohan 4 , Behzad Mansoori 3 , Jafar Soleymani 5 , Jafar Ezzati Nazhad Dolatabadi 6 , Michael R Hamblin 7
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Co-delivery of therapeutic agents and small interfering RNA (siRNA) can be achieved by a suitable nanovehicle. In this work, the solubility and bioavailability of curcumin (Cur) were enhanced by entrapment in a polyamidoamine (PAMAM) dendrimer, and a polyplex was formed by grafting Bcl-2 siRNA onto the surface amine groups to produce PAMAM-Cur/Bcl-2 siRNA nanoparticles (NPs). The synthesized polyplex NPs had a particle size of ∼180 nm, and high Cur loading content of ∼82 wt%. Moreover, the PAMAM-Cur/Bcl-2 siRNA NPs showed more effective cellular uptake, and higher inhibition of tumor cell proliferation compared to PAMAM-Cur nanoformulation and free Cur, due to the combined effect of co-delivery of Cur and Bcl-2 siRNA. The newly described PAMAM-Cur/Bcl-2 siRNA polyplex NPs could be a promising co-delivery nanovehicle.
中文翻译：
PAMAM树状聚合物共同递送姜黄素和Bcl-2 siRNA，以增强HeLa癌细胞的治疗功效。
治疗剂和小干扰RNA（siRNA）的共同递送可以通过合适的纳米载体来实现。在这项工作中，姜黄素（Cur）的溶解度和生物利用度通过截留在聚酰胺基胺（PAMAM）树枝状聚合物中而得到增强，并且通过将Bcl-2 siRNA嫁接到表面胺基上形成PAMAM-Cur / Bcl-2而形成了复合物siRNA纳米粒子（NPs）。合成的多链体纳米粒子的粒径约为180 nm，高Cur负载含量约为82 wt％。此外，与PAMAM-Cur纳米制剂和游离Cur相比，PAMAM-Cur / Bcl-2 siRNA NPs显示出更有效的细胞摄取，并且对肿瘤细胞增殖的抑制作用更高，这是由于Cur和Bcl-2共同递送的共同作用siRNA。新描述的PAMAM-Cur / Bcl-2 siRNA多聚体NPs可能是有前途的共同交付纳米车辆。
更新日期：2019-12-27
中文翻译：
PAMAM树状聚合物共同递送姜黄素和Bcl-2 siRNA，以增强HeLa癌细胞的治疗功效。
治疗剂和小干扰RNA（siRNA）的共同递送可以通过合适的纳米载体来实现。在这项工作中，姜黄素（Cur）的溶解度和生物利用度通过截留在聚酰胺基胺（PAMAM）树枝状聚合物中而得到增强，并且通过将Bcl-2 siRNA嫁接到表面胺基上形成PAMAM-Cur / Bcl-2而形成了复合物siRNA纳米粒子（NPs）。合成的多链体纳米粒子的粒径约为180 nm，高Cur负载含量约为82 wt％。此外，与PAMAM-Cur纳米制剂和游离Cur相比，PAMAM-Cur / Bcl-2 siRNA NPs显示出更有效的细胞摄取，并且对肿瘤细胞增殖的抑制作用更高，这是由于Cur和Bcl-2共同递送的共同作用siRNA。新描述的PAMAM-Cur / Bcl-2 siRNA多聚体NPs可能是有前途的共同交付纳米车辆。
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