BACKGROUND Previous studies have reported that exercise could improve the plasticity of hippocampal synapses. However, the effects of exercise on synapses in the hippocampus in Alzheimer's disease (AD) are not completely known. METHODS In this study, thirty 12-month-old male APP/PS1 double transgenic mice were randomly divided into a sedentary group (n = 15) and a running group (n = 15). Fifteen 12-month-old male wild-type littermates were assigned to the control group (n = 15). While running mice were assigned to treadmill running for four months, the control mice and sedentary mice did not run during the study period. After Morris water maze testing, five mice in each group were randomly selected for a stereological assessment of spinophilin-immunoreactive puncta in the CA1, CA2-3 and dentate gyrus (DG) of the hippocampus. RESULTS Morris water maze testing revealed that while the learning and memory abilities in sedentary APP/PS1 mice were significantly worse than those in wild-type control mice, the learning and memory abilities in running APP/PS1 mice were significantly better than those in sedentary APP/PS1 mice. The stereological results showed that the spinophilin-immunoreactive puncta numbers of the CA1, CA2-3 and DG in the hippocampus of sedentary APP/PS1 mice were significantly lower than those of wild-type control mice and that the numbers of these spines in the CA1, CA2-3 and DG in the hippocampus of running APP/PS1 mice were significantly higher than those of sedentary APP/PS1 mice. Moreover, a running-induced improvement in spatial learning and memory abilities was significantly correlated with running-induced increases in the spinophilin-immunoreactive puncta numbers in the CA1 and DG of the hippocampus. CONCLUSIONS Four-month treadmill exercise induced a significant improvement in spatial learning and memory abilities and a significant increase in the number of spinophilin-immunoreactive puncta of the CA1, CA2-3 and DG in the hippocampus of APP/PS1 mice. Running-induced improvements in spatial learning and memory abilities were significantly correlated with running-induced increases in the spinophilin-immunoreactive puncta numbers in the CA1 and DG of the hippocampus.

中文翻译：

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为期四个月的跑步机锻炼可防止APP / PS1转基因小鼠海马的空间学习和记忆能力下降以及海马中的亲纺素免疫反应性小点的丢失。

背景技术以前的研究已经报道运动可以改善海马突触的可塑性。但是，运动对阿尔茨海默氏病（AD）中海马突触的影响尚不完全清楚。方法在本研究中，将30只12个月大的雄性APP / PS1双转基因小鼠随机分为久坐组（n = 15）和跑步组（n = 15）。将15个12个月大的雄性野生同窝仔作为对照组（n = 15）。在将奔跑的小鼠指定在跑步机上跑步四个月时，对照小鼠和久坐的小鼠在研究期间没有奔跑。在进行莫里斯水迷宫测试后，每组随机选择五只小鼠进行海马CA1，CA2-3和齿状回（DG）中亲脂蛋白免疫反应性小点的体视学评估。结果莫里斯水迷宫测试显示，虽然久坐的APP / PS1小鼠的学习和记忆能力显着低于野生型对照小鼠，但奔跑的APP / PS1小鼠的学习和记忆能力明显优于久坐的APP / PS1小鼠。立体结果表明，久坐的APP / PS1小鼠海马中CA1，CA2-3和DG的亲脂蛋白免疫反应点数显着低于野生型对照小鼠，并且CA1中这些刺的数量奔跑的APP / PS1小鼠海马中的CA2-3和DG显着高于久坐的APP / PS1小鼠。而且，跑步诱发的空间学习和记忆能力的改善与跑步诱发的海马CA1和DG中的亲脂蛋白免疫反应点数显着相关。结论为期四个月的跑步机运动在APP / PS1小鼠的海马中诱导了空间学习和记忆能力的显着改善，并显着增加了CA1，CA2-3和DG的亲丝蛋白免疫反应点的数量。跑步诱导的空间学习和记忆能力的改善与跑步诱导的海马CA1和DG中亲脂蛋白免疫反应点数的增加显着相关。结论为期四个月的跑步机运动在APP / PS1小鼠的海马中诱导了空间学习和记忆能力的显着改善，并显着增加了CA1，CA2-3和DG的亲丝蛋白免疫反应点的数量。跑步诱导的空间学习和记忆能力的改善与跑步诱导的海马CA1和DG中亲脂蛋白免疫反应点数的增加显着相关。结论为期四个月的跑步机运动在APP / PS1小鼠的海马中诱导了空间学习和记忆能力的显着改善，并显着增加了CA1，CA2-3和DG的亲丝蛋白免疫反应点的数量。跑步诱导的空间学习和记忆能力的改善与跑步诱导的海马CA1和DG中亲脂蛋白免疫反应点数的增加显着相关。