This research evaluated the anxiolytic and antidepressant-like effects of a hydroethanolic extract from the leaves of Annona coriacea (EHFAC) and caffeic acid (CA). Mice were intraperitoneally treated with saline, EHFAC (1, 10, 20 mg/kg) or CA (0.15 mg/kg) and subject to the elevated plus-maze, open field, rota-rod, forced swimming and reserpine-induced akinesia tests. Pro-convulsant and anticholinergic effects were also evaluated. EHFAC presented anxiolytic-like effect on the elevated plus-maze, which was partially reversed by flumazenil. A similar effect was observed with CA. In the forced swimming test, EHFAC and CA reduced the immobility time of mice; such effect was potentiated when EHFAC or CA were associated with imipramine, bupropion and fluoxetine. The antidepressant-like effect was reinforced as EHFAC partially reversed the reserpine-induced akinesia. In addition, a pre-treatment with EHFAC and CA did not decrease the latency to 1st seizure of animals that received a sub-convulsive dose of PTZ, nor reduced the intensity of oxotremorine-induced tremors. Taken together, the results indicate that EHFAC and CA have anxiolytic and antidepressant-like effects, which involve important neurotransmitter systems, such as GABAergic and monoaminergic ones, being devoid of side effects, commonly associated with classical psychotropic drugs.

中文翻译：

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Annona coriacea（Mart。）和咖啡酸在小鼠中的抗焦虑和抗抑郁样作用。

这项研究评估了来自Annona coriacea（EHFAC）和咖啡酸（CA）叶片的氢乙醇提取物的抗焦虑和抗抑郁样作用。用盐水，EHFAC（1、10、20 mg / kg）或CA（0.15 mg / kg）腹膜内治疗小鼠，并进行高迷宫，开阔视野，旋转杆，强迫游泳和利血平诱导的运动障碍测试。还评估了前惊厥和抗胆碱能的作用。EHFAC对升高的迷宫呈抗焦虑样作用，氟马西尼可部分逆转。用CA观察到类似的效果。在强迫游泳试验中，EHFAC和CA减少了小鼠的不动时间。当EHFAC或CA与丙咪嗪，安非他酮和氟西汀联合使用时，这种作用会增强。当EHFAC部分逆转利血平诱导的运动障碍时，抗抑郁药样作用得到增强。此外，用EHFAC和CA进行预处理不会降低惊厥剂量PTZ的动物第一次癫痫发作的潜伏期，也不会降低氧代雷莫林诱发的震颤的强度。两者合计，结果表明EHFAC和CA具有抗焦虑和抗抑郁样作用，其中涉及重要的神经递质系统，例如GABA能和单胺能，没有副作用，通常与经典的精神药物有关。