Considering the widespread contaminations of food products with mycotoxins, it is important to develop, robust, time‐ and cost‐effective detection methods. We developed and optimized an immunoassay using a continuous flow system for the detection of zearalenone (ZEN). The assay was performed in a flow mode using an automated sequential injection system. Time for an assay cycle was 18 Min. Under optimal conditions, the limit for quantification for ZEN was 0.40 µg L⁻¹ with a linear dependency between concentration and signal amplitude between 0.10 and 10 µg L⁻¹. The assay proved to be robust and reliable with 13% relative standard deviation between measurements. By dissociating the antigen–antibody complex using a regeneration solution, we showed 50 times reusability of the immobilized antibodies without affecting the antigen‐binding properties.

中文翻译：

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用于连续检测玉米赤霉烯酮的基于流量的自动分光光度免疫分析仪的开发。

考虑到食品中真菌毒素的广泛污染，开发可靠，时间和成本效益高的检测方法非常重要。我们开发并优化了使用连续流动系统检测玉米赤霉烯酮（ZEN）的免疫测定方法。使用自动顺序注射系统以流动模式进行测定。测定周期的时间为18分钟。在最佳条件下，ZEN的定量限为0.40 µg L ^-1，浓度与信号幅度之间线性相关，介于0.10和10 µg L ^-1之间。该方法被证明是可靠且可靠的，测量之间的相对标准偏差为13％。通过使用再生溶液解离抗原-抗体复合物，我们发现固定化抗体的可重复使用性是其50倍，而不会影响抗原结合特性。