As a type of reactive oxygen species (ROS), hydroxyl radical (·OH) is closely associated with many kinds of diseases. The present study aimed to develo p a novel OH fluorescent probe based on coumarin, a new compound that has not been previously reported. This probe exhibited good linear range and selectivity for ·OHl, and is able to avoid interference from some metal ions and other kinds of ROS (H2 O2 , O2 .- , 1 O2 , and HClO). Meanwhile, this probe has been used to evaluate the ·OH-scavenging efficiency of different compounds, such as isopropyl alcohol, cytosine, uracil, Tempo, Glutathione (GSH), and dimethyl sulfoxide (DMSO). Therefore, the present study shows that this probe not only can effectively measure the level of ·OH, but also can assess the ·OH-scavenging efficiency of different compounds. Furthermore this current study suggested that following further optimization, this probe may be potentially applied in the diagnosis of oxidative stress in human body.

中文翻译：

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基于香豆素的荧光探针用于羟基自由基检测的合成和活性。

羟基自由基（·OH）作为一种活性氧（ROS），与多种疾病密切相关。本研究旨在开发一种基于香豆素的新型OH荧光探针，该香豆素是以前未曾报道过的新化合物。该探针对·OHl表现出良好的线性范围和选择性，并且能够避免受到某些金属离子和其他种类的ROS（H2 O2，O2-。，1 O2和HClO）的干扰。同时，该探针已用于评估不同化合物（例如异丙醇，胞嘧啶，尿嘧啶，tempo，谷胱甘肽（GSH）和二甲基亚砜（DMSO））的·OH清除效率。因此，本研究表明该探针不仅可以有效地测量·OH的水平，而且可以评估不同化合物的·OH清除效率。