Using plant bio-components for Designing green metal nanoparticles was considered as one of the most important methods in nanomedical application field due to their eco-friendly, cheap source, easily obtainable and having a high detection result. In this report, we fabricated eco-friendly engineering and cost-effective technique for green selenium nanoparticles from 0.01 M H₂SeO₃ solution using Asteriscus graveolens leaves extract as reducing and a capping agent at ambient temperature. Spectral techniques have been used to identify the formatted Selenium nanoparticles such as UV–Vis, pH, XPs, FT-IR, XRD, LDS, Z.P, EDS, TEM and AFM spectroscopy, which showed a size of 20 nm with spherical shape. Herein, the multi-effect of decorated Se-NPs surface have been evaluated, firstly on the hemolysis that showed completely hemocompatibility. Cytotoxicity assay showed that Se-NPs have a high selective effect on the HepG2 apoptosis and which proved by phase-contrast microscopy. Furthermore, the effect of nanoparticles on the action of the mechanism internal revealed that Se-NPs significantly and rapidly increased the level of reactive oxygen species and lipid peroxidation, while caused decreased the potential of mitochondrial membrane and glutathione level, which they together responsible on regulating the HepG2 cells fate. Furthermore, Flow cytometry analysis gave high values about S and G2/M phases of cell cycle resulting from Se-NPs effectiveness. In the end, with all the recorded information that has been measured in this study, this report provides a suitable and effective pathway for the green fabrication of Se-NPs decorated by biomolecules having high anticancer inhibited.

利用植物生物成分设计绿色金属纳米粒子由于其环保，廉价，易于获得且检测结果高等优点，被认为是纳米医学应用领域最重要的方法之一。在这份报告中，我们制作的环保工程和成本效益的技术，绿色纳米硒0.01 MH ₂的SeO ₃采用溶液Asteriscus芸在室温下将提取物用作还原剂和封端剂。光谱技术已用于鉴定格式化的硒纳米颗粒，例如UV-Vis，pH，XPs，FT-IR，XRD，LDS，ZP，EDS，TEM和AFM光谱，其球形大小为20 nm。本文中，已评价了修饰的Se-NPs表面的多重效果，首先是在表现出完全血液相容性的溶血作用上。细胞毒性试验表明，Se-NPs对HepG2细胞凋亡具有较高的选择性，并已通过相差显微镜证实。此外，纳米粒子对内部机理的作用表明，硒纳米颗粒显着快速增加了活性氧和脂质过氧化的水平，同时导致线粒体膜电位和谷胱甘肽水平降低，他们共同负责调节HepG2细胞的命运。此外，流式细胞仪分析给出了由Se-NPs有效性导致的细胞周期S和G2 / M期的高价值。最后，利用本研究中测量到的所有记录信息，该报告为绿色制备Se-NPs提供了一条合适而有效的途径，该Se-NPs被具有高度抗癌抑制作用的生物分子修饰。