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Active fluidic chip produced using 3D-printing for combinatorial therapeutic screening on liver tumor spheroid.
Biosensors and Bioelectronics ( IF 10.7 ) Pub Date : 2019-12-26 , DOI: 10.1016/j.bios.2019.111966
Yibo Feng 1 , Bingquan Wang 1 , Yin Tian 2 , Hao Chen 3 , Yonggang Liu 2 , Haiming Fan 3 , Kaige Wang 1 , Ce Zhang 1
Affiliation  

Known for their capabilities in automated fluid manipulation, microfluidic devices integrated with pneumatic valves are broadly used for researches in life science and clinical practice. The application is, however, hindered by the high cost and overly complex fabrication procedure. Here, we present an approach for fabricating molds of active fluidic devices using a benchtop 3D printer and a simple 2-step protocol (i.e. 3D printing and polishing). The entire workflow can be completed within 6 h, costing less than US$ 5 to produce all necessary templates for PDMS replica molding, which have smooth surface and round-shaped pneumatic valve structures. Moreover, 3D printing can create unique bespoke on-off objects of a wide range of dimensions. The millimeter- and centimeter-sized features allow examination of large-scale biological samples. Our results demonstrate that the 3D-printed active fluidic device has valve control capacities on par with those made by photolithography. Controlled nutrients and ligands delivery by on-off active valves allows generation of dynamic signals mimicking the ever-changing environmental stimuli, and combinatorial/sequential drug inputs for therapeutic screening on liver tumor spheroid. We believe that the proposed methodology can pave the way for integration of active fluidic systems in research labs, clinical settings and even household appliances for a broad range of application.

中文翻译:

使用3D打印生产的活性流体芯片,用于肝肿瘤球体的组合治疗筛选。

集成了气动阀的微流体设备以其在自动流体处理方面的能力而著称,被广泛用于生命科学和临床实践的研究。然而,该应用由于高成本和过于复杂的制造过程而受到阻碍。在这里,我们介绍一种使用台式3D打印机和简单的两步协议(即3D打印和抛光)制造有源流体设备模具的方法。整个工作流程可在6小时内完成,花费不到5美元即可生产出PDMS复制品成型所需的所有模板,这些模板具有光滑的表面和圆形气动阀结构。此外,3D打印可以创建各种尺寸的独特定制开关对象。毫米和厘米大小的功能部件可以检查大规模的生物样本。我们的结果表明,3D打印的主动流体装置具有与光刻技术相同的阀门控制能力。通过开关式主动阀控制营养和配体的输送,可产生动态信号,模拟不断变化的环境刺激,并提供组合/顺序药物输入以用于肝肿瘤球体的治疗性筛查。我们相信,所提出的方法学可以为在研究实验室,临床环境甚至家用电器中实现广泛应用的有源流体系统集成铺平道路。通过开关式主动阀控制营养和配体的输送,可产生动态信号,模拟不断变化的环境刺激,并提供组合/顺序药物输入以用于肝肿瘤球体的治疗性筛查。我们认为,所提出的方法学可以为在研究实验室,临床环境甚至家用电器中实现广泛应用的有源流体系统集成铺平道路。通过开关式主动阀控制营养和配体的输送,可产生动态信号,模拟不断变化的环境刺激,并提供组合/顺序药物输入以用于肝肿瘤球体的治疗性筛查。我们认为,所提出的方法学可以为在研究实验室,临床环境甚至家用电器中实现广泛应用的有源流体系统集成铺平道路。
更新日期:2019-12-27
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