Embryo transfer (ET) of 20 porcine expanded blastocysts (ExBs) vitrified and warmed (VW) by open pulled straw (OPS) to a recipient allows stable piglet production. The efficiency of artificial insemination (AI) prior to ET of 10 VW ExBs for piglet production was investigated. For one trial, 10-15 VW ExBs from single donor were assigned, 10 were used for ET and the remains were assessed for their in vitro viability. In the non-AI/ET group, 10 were transferred to each of five recipients. As AI/ET group, 10 were transferred to each of five recipients after AI. In AI/non-ET group, only AI was performed to seven gilts. In the non-AI/ET group, the pregnancy rate was 40%, but none of them farrowed. In the AI/ET group, all recipients produced piglets. Four (80.0%) delivered piglets from transferred VW ExBs. The survival rate of VW ExBs to term was 20.0% (10/50). In the AI/non-ET group, six of the seven gilts farrowed. There was no difference in in vitro viability between the non-AI/ET and AI/ ET groups (62.5% and 68.3%, respectively). AI prior to ET can be an appropriate way to maintain pregnancy and assist the development of a low number of VW ExBs to term.

中文翻译：

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在通过开放式吸管 (OPS) 方法转移有限数量的玻璃化和加热猪胚胎之前进行人工授精对其分娩存活能力的影响。

通过开放式吸管 (OPS) 将 20 个猪膨胀囊胚 (ExB) 玻璃化并加热 (VW) 的胚胎移植 (ET) 至受体，可实现稳定的仔猪生产。研究了 10 头 VW ExB 在 ET 前人工授精 (AI) 对仔猪生产的效率。在一项试验中，分配了来自单个供体的 10-15 个 VW ExB，其中 10 个用于 ET，并评估了遗体的体外生存能力。在非 AI/ET 组中，将 10 个转移到五个接受者中的每一个。作为 AI/ET 组，在 AI 后将 10 个转移给五个接受者中的每一个。在 AI/非 ET 组中，仅对 7 头母猪进行了 AI。在非 AI/ET 组中，妊娠率为 40%，但均未分娩。在 AI/ET 组中，所有接受者都生产了仔猪。四只 (80.0%) 从转移的 VW ExB 中分娩仔猪。大众 ExB 的存活率为 20。0% (10/50)。在 AI/非 ET 组中，7 头后备母猪中有 6 头产仔。非 AI/ET 组和 AI/ET 组之间的体外存活率没有差异（分别为 62.5% 和 68.3%）。在 ET 之前进行 AI 可能是维持妊娠和帮助将少量 VW ExB 发育至足月的适当方法。