Mechanisms of Chaperones as Active Assistant/Protector for Proteins: Insights from NMR Studies,Chinese Journal of Chemistry

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Mechanisms of Chaperones as Active Assistant/Protector for Proteins: Insights from NMR Studies
Chinese Journal of Chemistry ( IF 5.5 ) Pub Date : 2020-02-21 , DOI: 10.1002/cjoc.201900441
Yunfei Hu _{1,

2} , Conggang Li _{1,

2} , Lichun He _{1,

2} , Changwen Jin ₃ , Maili Liu _{1,

2}

Affiliation

Molecular chaperones are diverse families of proteins that play key roles in protein homeostasis. They assist the folding of client proteins or prevent them from irreversible aggregation under stress conditions. Diverse chaperone families contribute to different aspects of protein homeostasis by interacting with a wide range of client proteins. Despite the vital roles of chaperones in cell survival, the molecular mechanisms underlying chaperone functions remain elusive, due to the non‐specificity of chaperone‐client interactions and the intrinsic flexibility of the clients. Our understanding of the chaperone functional mechanisms, especially regarding chaperone‐client interactions, has greatly expanded in recent years, thanks to the significant contribution from various NMR studies. Solution NMR methods have unique advantages in characterizing disordered protein structures, detecting weak and non‐specific interactions, and probing conformational dynamics of proteins and protein complexes, etc., and therefore are especially powerful in the studies of chaperone structure‐function relationships. In this review, we summarize some of the current knowledge of molecular chaperones, with emphasis on common features of chaperone‐client interactions and examples on a number of specific systems in which solution NMR methods were used to provide essential insights into their functional mechanisms.

中文翻译：

伴侣蛋白作为蛋白质活性助剂/保护剂的机理：NMR研究的启示

分子伴侣是蛋白质的各种家族，它们在蛋白质稳态中起关键作用。它们有助于客户蛋白质的折叠或防止它们在压力条件下不可逆的聚集。不同的伴侣家族通过与广泛的客户蛋白质相互作用，促进了蛋白质稳态的不同方面。尽管伴侣分子在细胞存活中起着至关重要的作用，但由于伴侣与客户之间相互作用的非特异性和客户的内在灵活性，伴侣分子功能的分子机制仍然难以捉摸。近年来，由于各种NMR研究的巨大贡献，我们对伴侣功能机制的理解，尤其是在伴侣与客户之间的相互作用上，有了很大的发展。等，因此在伴侣结构与功能关系的研究中特别有力。在这篇综述中，我们总结了分子伴侣的一些最新知识，重点介绍了伴侣-客户相互作用的共同特征，并举例说明了许多使用溶液NMR方法提供其功能机理的特定系统的特定系统。

更新日期：2020-02-21

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