Polyvinyl alcohol/carboxymethylcellulose/silver sulfadiazine composite nanofibers (PVA/CMC/SSD) containing different proportions of each ingredient were prepared by electrospinning and characterized by Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermo-gravimetric analysis (TGA), powder X-ray diffraction analysis (XRD), atomic absorption spectroscopy (AAS), and ultraviolet-visible (UV-Vis) spectroscopy. The obtained nanofibers were uni-axially formed with randomly oriented longitudinal axes without entanglement, and their mean diameters ranged from 160 to 210 ran. Silver sulfadiazine, on the other hand, was appeared as a non-uniform dispersion of spherical nanoparticles ranging from 35 to 75 nm over the surface of nanofibers. Efficacy of the silver sulfadiazine loaded nanofibers for treatment of wounds caused be excision in rabbits in a period of 14 days was evaluated and it was found that the extent of skin repair in the applied dressing depends on the total Ag content. The dressing composed of 73.6:24.6:1.8 weight percent of PVA/CMC/SSD provided the best result in comparison with a control dressing made form PVA/CMC (88.8:11.2 %). In vitro cell viability of fibers on HSF-PI 18 fibroblast like cells was assessed by MTT assay. Antibacterial activity of the prepared nanofibers was also evaluated against gram-negative Escherichia coli and Pseudomonas aeroginosa, and gram-positive Staphylococcus aureus. PVA/CMC/SSD samples, was shown to have significant inhibition effect in comparison with drug references including penicillin G, chloramphenicol and erythromycin as positive controls (P0.05).

中文翻译：

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载有磺胺嘧啶银的PVA / CMC纳米纤维用于治疗切除伤口

通过电纺丝制备包含每种成分不同比例的聚乙烯醇/羧甲基纤维素/磺胺嘧啶银复合纳米纤维（PVA / CMC / SSD），并通过傅里叶变换红外光谱（FTIR），扫描电子显微镜（SEM），热重分析（ TGA），粉末X射线衍射分析（XRD），原子吸收光谱（AAS）和紫外可见光谱（UV-Vis）。所获得的纳米纤维是在无缠结的情况下以随机取向的纵轴单轴形成的，其平均直径范围为160至210纳米。另一方面，磺胺嘧啶银表现为球形纳米颗粒在纳米纤维表面上的不均匀分散，范围从35到75 nm。评价了磺胺嘧啶银负载的纳米纤维在14天内治疗兔子的伤口所引起的伤口的功效，发现敷料中皮肤修复的程度取决于总的Ag含量。与由PVA / CMC制成的对照敷料（88.8：11.2％）相比，由73.6：24.6：1.8重量百分比的PVA / CMC / SSD组成的敷料提供了最好的结果。通过MTT测定评估纤维在HSF-PI 18成纤维细胞样细胞上的体外细胞生存力。还评估了制备的纳米纤维对革兰氏阴性菌的抗菌活性。与由PVA / CMC制成的对照敷料相比（88.8：11.2％），8％的PVA / CMC / SSD提供了最好的结果。通过MTT测定评估纤维在HSF-PI 18成纤维细胞样细胞上的体外细胞生存力。还评估了制备的纳米纤维对革兰氏阴性菌的抗菌活性。与由PVA / CMC制成的对照敷料相比（88.8：11.2％），8％的PVA / CMC / SSD提供了最好的结果。通过MTT测定评估纤维在HSF-PI 18成纤维细胞样细胞上的体外细胞生存力。还评估了制备的纳米纤维对革兰氏阴性菌的抗菌活性。大肠杆菌和铜绿假单胞菌，以及革兰氏阳性金黄色葡萄球菌。与以青霉素G，氯霉素和红霉素为阳性对照的药物参考相比，PVA / CMC / SSD样品具有明显的抑制作用（P 0.05）。