Thermogelling chitosan-based polymers for the treatment of oral mucosa ulcers.,Biomaterials Science

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Thermogelling chitosan-based polymers for the treatment of oral mucosa ulcers.
Biomaterials Science ( IF 5.8 ) Pub Date : 2020-01-09 , DOI: 10.1039/c9bm01754b
Zheng Luo ₁ , Kun Xue , Xikui Zhang , Jason Y C Lim , Xiyu Lai , David James Young , Zhong-Xing Zhang , Yun-Long Wu , Xian Jun Loh

Affiliation

Current treatments for oral mucosa-related ulcers use drugs to relieve pain and promote healing, but rarely consider drug resistance to bacterial infection in the microenvironment of the oral cavity or the prevention of bleeding from gingival mucosa ulcers. We herein report an injectable, thermogelling chitosan-based system to address these concerns. An aqueous solution of chitosan-based conjugates (chitosan-g-poly(N-isopropylacrylamide) [CS-g-PNIPAAM] including 1a [CS-g-PNIPAAM with less PNIPAAM] and 1b [CS-g-PNIPAAM with more PNIPAAM], and chitosan-g-poly(N-isopropylacrylamide)-g-polyacrylamide [CS-g-PNIPAAM-g-PAM] 3) could reversibly form semi-solid gels at physiological temperatures for easy application to oral cavity ulcer sites by injection. The chitosan-based conjugate thermogels prepared could inhibit both Gram-positive and Gram-negative bacteria and the two with higher chitosan and poly(N-isopropylacrylamide) contents (1a and 1b) promoted proliferation of gingival fibroblasts in vitro. These two thermogels also exhibited improved blood clotting in an in vivo rat study. Thermogels 1a and 1b effectively promoted ulcer healing and shortened ulcer healing times in an oral gingival mucosa ulcer model using Sprague Dawley (SD) rats. These thermogels showed no obvious toxicity to the main organs of SD rats undergoing gingival ulcer treatment. These results suggest that this antibacterial biomaterial could be a promising injectable therapeutic agent for the treatment for oral mucosa ulcers.

中文翻译：

基于壳聚糖的热凝胶聚合物，用于治疗口腔粘膜溃疡。

口腔粘膜相关溃疡的当前疗法使用药物来减轻疼痛并促进愈合，但是很少考虑在口腔的微环境中对细菌感染的耐药性或牙龈粘膜溃疡的出血的预防。我们在此报告了一种可注射的基于热凝胶的壳聚糖系统，以解决这些问题。壳聚糖基偶联物（壳聚糖-g-聚（N-异丙基丙烯酰胺）[CS-g-PNIPAAM]的水溶液，其中包括1a [CS-g-PNIPAAM的PNIPAAM较少]和1b [CS-g-PNIPAAM的PNIPAAM] ，而壳聚糖-g-聚（N-异丙基丙烯酰胺）-g-聚丙烯酰胺[CS-g-PNIPAAM-g-PAM] 3）可以在生理温度下可逆地形成半固体凝胶，易于通过注射应用于口腔溃疡部位。制备的基于壳聚糖的共轭热凝胶可以抑制革兰氏阳性细菌和革兰氏阴性细菌，并且壳聚糖和聚（N-异丙基丙烯酰胺）含量较高的两种细菌（1a和1b）可促进牙龈成纤维细胞的体外增殖。在体内大鼠研究中，这两种热凝胶还显示出改善的血液凝结。在使用Sprague Dawley（SD）大鼠的口腔齿龈粘膜溃疡模型中，热凝胶1a和1b有效地促进了溃疡的愈合并缩短了溃疡的愈合时间。这些热凝胶对接受牙龈溃疡治疗的SD大鼠的主要器官没有明显的毒性。这些结果表明，这种抗菌生物材料可能是用于治疗口腔粘膜溃疡的有前途的可注射治疗剂。

更新日期：2020-03-03

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