BACKGROUND The identification of Trypanosoma cruzi and blood-meal sources in synanthropic triatomines is important to assess the potential risk of Chagas disease transmission. We identified T. cruzi infection and blood-meal sources of triatomines caught in and around houses in the state of Bahia, northeastern Brazil, and mapped the occurrence of infected triatomines that fed on humans and domestic animals. METHODS Triatominae bugs were manually captured by trained agents from the Epidemiologic Surveillance team of Bahia State Health Service between 2013 and 2014. We applied conventional PCR to detect T. cruzi and blood-meal sources (dog, cat, human and bird) in a randomized sample of triatomines. We mapped triatomine distribution and analyzed vector hotspots with kernel density spatial analysis. RESULTS In total, 5906 triatomines comprising 15 species were collected from 127 out of 417 municipalities in Bahia. The molecular analyses of 695 triatomines revealed a ~10% T. cruzi infection rate, which was highest in the T. brasiliensis species complex. Most bugs were found to have fed on birds (74.2%), and other blood-meal sources included dogs (6%), cats (0.6%) and humans (1%). Trypanosoma cruzi-infected triatomines that fed on humans were detected inside houses. Spatial analysis showed a wide distribution of T. cruzi-infected triatomines throughout Bahia; triatomines that fed on dogs, humans, and cats were observed mainly in the northeast region. CONCLUSIONS Synanthropic triatomines have a wide distribution and maintain the potential risk of T. cruzi transmission to humans and domestic animals in Bahia. Ten species were recorded inside houses, mainly Triatoma sordida, T. pseudomaculata, and the T. brasiliensis species complex. Molecular and spatial analysis are useful to reveal T. cruzi infection and blood-meal sources in synanthropic triatomines, identifying areas with ongoing threat for parasite transmission and improving entomological surveillance strategies.

中文翻译：

---

在巴西巴伊亚州，锥虫锥虫感染的三氢番茄红素分布广泛。

背景技术识别人类合生三氢嘧啶中的克氏锥虫和血粉来源对于评估南美锥虫病传播的潜在风险很重要。我们确定了巴西东北部巴伊亚州房屋内及附近房屋中捕获的锥虫的T. cruzi感染和血粉来源，并绘制了以人类和家畜为食的被感染的三角藻的发生情况。方法在2013年至2014年之间，由巴伊亚州卫生局的流行病学监测小组的受过训练的代理人手动捕获了三角藻虫。我们应用常规PCR随机检测了克氏锥虫和血粉来源（狗，猫，人和鸟）样品中的三atomines。我们绘制了三氢精氨酸分布图，并使用核密度空间分析法分析了矢量热点。结果总计 从巴伊亚州的417个城市中的127个中收集了5906种三氟精氨酸，共15种。对695种三氢嘧啶的分子分析表明，克鲁维氏锥虫感染率约为10％，在巴西锥虫物种复合体中最高。发现大多数臭虫以鸟类为食（74.2％），其他血粉来源包括狗（6％），猫（0.6％）和人类（1％）。在房屋内检测到以人类为食的克氏锥虫感染的三氢番茄红素。空间分析表明，巴伊亚州的克鲁索氏菌感染的三氢番茄红素分布广泛。以狗，人和猫为食的三氢嘧啶主要在东北地区被观察到。结论拟人三氢嘧啶分布广泛，并保持巴伊亚州克氏锥虫传播给人类和家畜的潜在风险。房屋内记录了十种 主要是鞭毛藻（Triatoma sordida），伪拟锥虫（T. pseudomaculata）和巴西纤毛虫（T. brasiliensis）物种复合体。分子和空间分析可用于揭示拟人三聚体中的克氏锥虫感染和血粉来源，确定存在寄生虫传播持续威胁的区域，并改进昆虫学监测策略。