Background Gastric cancer (GC) is one common cancer which occurs in the stomach leading to high mortality around the world. Long non-coding RNAs (lncRNAs) were found overexpressed or silenced in the occurrence and progression of multiple cancers including GC. Method The gene expression level in GC tissues and cells were analyzed by RT-qPCR. CCK-8, colony formation, flow cytometry and transwell assays were performed for the function analysis of HLA complex group 11 (HCG11). The mechanism study for HCG11 was conducted using RIP, RNA pull down and luciferase reporter assays. Results HCG11 was discovered highly expressed in GC tissues and cells. Depletion experiments were used to evaluate HCG11 silence on cell proliferation, migration and apoptosis. Moreover, Wnt signaling pathway was found as a tumor promoter in GC. RIP assay, RNA pull down assay and luciferase reporter assay were performed to illustrate the relationship of HCG11, miR-1276 and CTNNB1. Rescue assays revealed that HCG11/miR-1276/CTNNB1 axis regulated the incidence and development of GC. Tumor formation in mice proved that HCG11 was negatively correlated with miR-1276 and had positively correlation with CTNNB1. Conclusion Overall, HCG11 accelerated proliferation and migration in GC through miR-1276/CTNNB1 and Wnt signaling pathway, revealing that HCG11 could be a brand new target for GC.

中文翻译：

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LncRNA HCG11通过靶向miR-1276/CTNNB1并激活Wnt信号通路促进胃癌的增殖和迁移。

背景胃癌（GC）是一种常见的癌症，发生在胃部，导致全世界的高死亡率。在包括 GC 在内的多种癌症的发生和进展中，发现长链非编码 RNA (lncRNA) 过表达或沉默。方法通过RT-qPCR分析GC组织和细胞中的基因表达水平。对 HLA 复合物组 11 (HCG11) 的功能进行 CCK-8、集落形成、流式细胞术和 transwell 测定。HCG11 的机制研究是使用 RIP、RNA pull down 和荧光素酶报告基因分析进行的。结果发现HCG11在GC组织和细胞中高表达。耗竭实验用于评估 HCG11 沉默对细胞增殖、迁移和凋亡的影响。此外，Wnt 信号通路被发现是 GC 中的肿瘤启动子。RIP 检测，进行 RNA pull down 测定和荧光素酶报告基因测定以说明 HCG11、miR-1276 和 CTNNB1 的关系。救援分析显示 HCG11/miR-1276/CTNNB1 轴调节 GC 的发生和发展。小鼠肿瘤形成证明HCG11与miR-1276呈负相关，与CTNNB1呈正相关。结论 总体而言，HCG11通过miR-1276/CTNNB1和Wnt信号通路加速GC中的增殖和迁移，揭示HCG11可能成为GC的全新靶点。小鼠肿瘤形成证明HCG11与miR-1276呈负相关，与CTNNB1呈正相关。结论 总体而言，HCG11通过miR-1276/CTNNB1和Wnt信号通路加速GC中的增殖和迁移，揭示HCG11可能成为GC的全新靶点。小鼠肿瘤形成证明HCG11与miR-1276呈负相关，与CTNNB1呈正相关。结论 总体而言，HCG11通过miR-1276/CTNNB1和Wnt信号通路加速GC中的增殖和迁移，揭示HCG11可能成为GC的全新靶点。