Proteomic analysis of foodborne pathogen Listeria monocytogenes after treatment with three disinfectants based on ammonium salts of pyridoxal oxime (POD) reveal perturbation of cellular processes. These inhibitors caused disturbance in the synthesis of plasma membrane proteins and cell wall proteoglycans. Some of key proteins and proteoglycans from these two groups that are important for bacterial growth are down-regulated. Additionally, we demonstrated that the main bacterial toxin Listeriolysin O (LLO) is significantly down-regulated after treatment with each of three investigated inhibitors. These investigations confirm already postulated mechanism of action of POD-based inhibitors that results in disturbance of key cell surface proteins and proteoglycans in Gram-positive bacteria. Additionally, the use of some proteins such as LLO, as potential biomarker candidates of food poisoning with this bacterium is discussed.

食源性单核细胞增生李斯特氏菌的蛋白质组学分析用基于吡ido醛肟（POD）铵盐的三种消毒剂处理后，发现细胞过程受到干扰。这些抑制剂在质膜蛋白和细胞壁蛋白聚糖的合成中引起干扰。这两个对细菌生长很重要的关键蛋白质和蛋白聚糖的一些蛋白被下调。此外，我们证明了三种研究的抑制剂中的每一种在治疗后主要细菌毒素李斯特菌溶血素O（LLO）均显着下调。这些研究证实了已经假定的基于POD的抑制剂的作用机理，该机理导致了革兰氏阳性细菌中关键细胞表面蛋白和蛋白聚糖的紊乱。此外，使用某些蛋白质（例如LLO，