Shape characterization and discrimination of single nanoparticles using solid-state nanopores.,Analyst

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Shape characterization and discrimination of single nanoparticles using solid-state nanopores.
Analyst ( IF 3.6 ) Pub Date : 2020-01-10 , DOI: 10.1039/c9an01889a
Wei Si ₁ , Jingjie Sha , Qianyi Sun , Zhen He , Liang Wu , Chang Chen , Shuhong Yu , Yunfei Chen

Affiliation

Resistive pulse sensing with nanopores is expected to enable identification and analysis of nanoscale objects in ionic solutions. However, there is currently no remarkable method to characterize the three-dimensional shape of charged biomolecules or nanoparticles with low-cost and high-throughput. Here we demonstrate the sensing capability of solid-state nanopores for shape characterization of single nanoparticles by monitoring the ionic current blockades during their electrophoretic translocation through nanopores. By using nanopores that are a bit larger than the particles, shape characterization of both spherical and cubic silver nanoparticles is successfully realized due to their rapid rotation with respect to the pore axis, which is further validated by our all-atom molecular dynamics simulations. The single-molecule approach based on nanopores will allow people to measure the dimension and to characterize the shape of single nanoparticles or proteins simultaneously in real time, which is significant for its potential application in investigation of structural biology and proteomics in the near future.

中文翻译：

使用固态纳米孔的单个纳米颗粒的形状表征和鉴别。

具有纳米孔的电阻式脉冲传感有望实现离子溶液中纳米级物体的识别和分析。但是，目前尚无卓越的方法来表征具有低成本和高通量的带电生物分子或纳米颗粒的三维形状。在这里，我们通过监测离子电流在其通过纳米孔的电泳移位过程中的阻滞作用，证明了固态纳米孔对单个纳米颗粒的形状表征的感测能力。通过使用比粒子大一点的纳米孔，由于球形和立方银纳米粒子相对于孔轴的快速旋转，成功实现了球形和立方银纳米粒子的形状表征，这已通过我们的全原子分子动力学模拟得到了进一步验证。

更新日期：2020-03-03

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