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One-Step Biotinylation of Cellulose Paper by Polymer Coating to Prepare a Paper-Based Analytical Device.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-01-08 , DOI: 10.1021/acs.analchem.9b04373
Kazuki Kaneko 1 , Manami Hara 1 , Takashi Nishino 1 , Tatsuo Maruyama 1
Affiliation  

Cellulose paper has strong potential as an analytical platform owing to its unique characteristics. In the present study, we investigated a procedure for functionalizing the surface of cellulose paper by dip-coating a mixture of a functional polymer and a perfluoroalkylated surfactant (surfactant 1). The functional polymer comprised a mixture of methyl methacrylate and poly(ethylene glycol) methacrylate monomers. The monomer ratio in the functional polymer affected the hydrophilicity and water absorbance of the cellulose paper after dip-coating. Furthermore, the presence of surfactant 1 during dip-coating promoted the surface segregation of poly(ethylene glycol) (PEG) moieties in the polymer, which enhanced the hydrophilicity, prevented nonspecific protein adsorption, and maintained the water absorbance of the dip-coated cellulose paper. Dip-coating with another functional polymer containing biotin groups produced a cellulose paper with a biotin-decorated surface in a one-step procedure. The displayed biotin groups immobilized avidin on the surface, and the PEG moieties in the polymer prevented nonspecific protein adsorption. We then immobilized a thrombin-binding DNA aptamer on the avidin-immobilized cellulose paper to prepare a paper-based analytical device. It is possible to visualize thrombin in model solutions and serum using the paper-based analytical device.

中文翻译:

通过聚合物涂层对纤维素纸进行一步生物素化,以制备纸基分析设备。

纤维素纸由于其独特的特性,具有作为分析平台的强大潜力。在本研究中,我们研究了通过浸涂功能性聚合物和全氟烷基化表面活性剂(表面活性剂1)的混合物使纤维素纸表面功能化的方法。功能聚合物包括甲基丙烯酸甲酯和聚(乙二醇)甲基丙烯酸酯单体的混合物。功能聚合物中的单体比例影响浸涂后纤维素纸的亲水性和吸水率。此外,浸涂过程中表面活性剂1的存在促进了聚合物中聚乙二醇(PEG)部分的表面偏析,从而增强了亲水性,防止了非特异性蛋白质的吸附,并保持了浸涂纤维素的吸水率纸。用另一种包含生物素基团的功能性聚合物进行浸涂,可以一步生产出具有生物素修饰表面的纤维素纸。展示的生物素基团将抗生物素蛋白固定在表面上,并且聚合物中的PEG部分阻止了非特异性蛋白质的吸附。然后,我们将抗凝血酶的DNA适体固定在抗生物素蛋白固定的纤维素纸上,以制备基于纸的分析装置。使用纸质分析仪可以可视化模型溶液和血清中的凝血酶。然后,我们将抗凝血酶的DNA适体固定在抗生物素蛋白固定的纤维素纸上,以制备基于纸的分析装置。使用纸质分析仪可以可视化模型溶液和血清中的凝血酶。然后,我们将抗凝血酶的DNA适体固定在抗生物素蛋白固定的纤维素纸上,以制备基于纸的分析装置。使用纸质分析仪可以可视化模型溶液和血清中的凝血酶。
更新日期:2020-01-08
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