当前位置：
X-MOL 学术
›
Anal. Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Yb-Based Nanoparticles with the Same Excitation and Emission Wavelength for Sensitive in Vivo Biodetection.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-01-08 , DOI: 10.1021/acs.analchem.9b04448 Na Wu 1 , Yuyang Gu 1 , Mengya Kong 1 , Qingyun Liu 1 , Shengming Cheng 1 , Yuwen Yang 2 , Wei Feng 1 , Fuyou Li 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-01-08 , DOI: 10.1021/acs.analchem.9b04448 Na Wu 1 , Yuyang Gu 1 , Mengya Kong 1 , Qingyun Liu 1 , Shengming Cheng 1 , Yuwen Yang 2 , Wei Feng 1 , Fuyou Li 1
Affiliation
|
Near-infrared luminescent emission has been widely used as a signal for biological detection with its high spatial resolution and fast response. Rare-earthdoped nanoparticle-dye composites have diverse advantages of a wide operation wavelength and remarkable light stability, while the application is limited by the low luminescence quantum yield of rare-earth nanoparticles. Hence, in this work, we use a singly Yb doped nanoparticle that has strong luminescence emission at 975 nm under excitation at the same wavelength as an energy donor to construct the detection system. An inner filter pair, composed of core-shell nanophosphor NaYF4/20%Yb@NaYF4 (1:2) nanocrystals (csYb) as a luminescent beacon and ClO--responsive cyanine dye Cy890 as a filtering agent, was designed as a model. With a time-gated detection mode, the nanocomposites realize the detection limit at 0.55 ppb as demonstrated in a ClO- detection trial. The csYb&Cy890 nanocomposites can also monitor ClO- by luminescence signals in both living cells and mice models.
中文翻译：
具有相同激发和发射波长的基于Yb的纳米粒子，可用于体内生物检测。
近红外发光发射以其高空间分辨率和快速响应而被广泛用作生物检测的信号。稀土掺杂纳米颗粒染料复合材料具有工作波长宽，光稳定性显着的多种优点，而其应用受到稀土纳米颗粒发光量子产率低的限制。因此，在这项工作中，我们使用掺杂了Yb的单个纳米粒子，该纳米粒子在与能量供体相同的波长激发下在975 nm处具有强发光发射，从而构建了检测系统。设计了一个内部过滤器模型作为模型，该内部过滤器模型由核壳纳米磷光体NaYF4 / 20％Yb @ NaYF4（1：2）纳米晶体（csYb）作为发光信标和ClO响应花青染料Cy890作为过滤剂组成。在定时门控模式下，如ClO检测试验所示，纳米复合材料实现了0.55 ppb的检测限。csYb＆Cy890纳米复合材料还可以通过活细胞和小鼠模型中的发光信号监测ClO-。
更新日期：2020-01-08
中文翻译：
具有相同激发和发射波长的基于Yb的纳米粒子，可用于体内生物检测。
近红外发光发射以其高空间分辨率和快速响应而被广泛用作生物检测的信号。稀土掺杂纳米颗粒染料复合材料具有工作波长宽，光稳定性显着的多种优点，而其应用受到稀土纳米颗粒发光量子产率低的限制。因此，在这项工作中，我们使用掺杂了Yb的单个纳米粒子，该纳米粒子在与能量供体相同的波长激发下在975 nm处具有强发光发射，从而构建了检测系统。设计了一个内部过滤器模型作为模型，该内部过滤器模型由核壳纳米磷光体NaYF4 / 20％Yb @ NaYF4（1：2）纳米晶体（csYb）作为发光信标和ClO响应花青染料Cy890作为过滤剂组成。在定时门控模式下，如ClO检测试验所示，纳米复合材料实现了0.55 ppb的检测限。csYb＆Cy890纳米复合材料还可以通过活细胞和小鼠模型中的发光信号监测ClO-。
京公网安备 11010802027423号