Molecular Wiring of a Mitochondrial Translational Feedback Loop.,Molecular Cell

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Molecular Wiring of a Mitochondrial Translational Feedback Loop.
Molecular Cell ( IF 14.5 ) Pub Date : 2019-12-26 , DOI: 10.1016/j.molcel.2019.11.019
Roger Salvatori ₁ , Kirsten Kehrein ₁ , Abeer Prakash Singh ₁ , Wasim Aftab ₂ , Braulio Vargas Möller-Hergt ₁ , Ignasi Forne ₃ , Axel Imhof ₃ , Martin Ott ₁

Affiliation

The mitochondrial oxidative phosphorylation system comprises complexes assembled from subunits derived from mitochondrial and nuclear gene expression. Both genetic systems are coordinated by feedback loops, which control the synthesis of specific mitochondrial encoded subunits. Here, we studied how this occurs in the case of cytochrome b, a key subunit of mitochondrial complex III. Our data suggest the presence of a molecular rheostat consisting of two translational activators, Cbp3-Cbp6 and Cbs1, which operates at the mitoribosomal tunnel exit to connect translational output with assembly efficiency. When Cbp3-Cbp6 is engaged in assembly of cytochrome b, Cbs1 binds to the tunnel exit to sequester the cytochrome b-encoding mRNA, repressing its translation. After mediating complex III assembly, binding of Cbp3-Cbp6 to the tunnel exit replaces Cbs1 and the bound mRNA to permit cytochrome b synthesis. Collectively, the data indicate the molecular wiring of a feedback loop to regulate synthesis of a mitochondrial encoded protein.

中文翻译：

线粒体翻译反馈环的分子布线。

线粒体的氧化磷酸化系统包括由衍生自线粒体和核基因表达的亚基组装而成的复合物。这两个遗传系统都由反馈环协调，该反馈环控制特定线粒体编码亚基的合成。在这里，我们研究了这种情况在细胞色素b（线粒体复合物III的关键亚基）的情况下如何发生。我们的数据表明存在由两个翻译活化剂Cbp3-Cbp6和Cbs1组成的分子变阻器，该活化剂在线粒体通道出口处运行，以连接翻译输出和装配效率。当Cbp3-Cbp6参与细胞色素b的组装时，Cbs1结合到隧道出口以隔离编码细胞色素b的mRNA，从而抑制其翻译。调解复杂的III装配体后，Cbp3-Cbp6绑定到隧道出口替换Cbs1和绑定的mRNA，以允许细胞色素b合成。总体而言，数据表明了反馈回路的分子布线，以调节线粒体编码蛋白的合成。

更新日期：2019-12-27

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