Silver nanoclusters stabilized with DNA scaffolds, with excellent physical and chemical properties, have emerged as a versatile tool in biomedical systems. Silver nanoclusters as a late-model nanomaterial with a small size less than 2 nm shows good stability and strong fluorescence. By using DNA with diverse sequence and structures, soluble DNA-templated silver nanoclusters are facilely prepared. They possess tunable fluorescence emission and are suitable with multifunctional designs. Extensive efforts have been put into the application potential of this biocompatible material for biosensing, bioimaging and therapy. Here we are committed to outline the recent advances of DNA-templated silver nanoclusters in biomedical application. The future prospect and challenges are then discussed considering rising progress. Though there is still a long way for illuminating the mechanism behind traits and conducting further clinical trials, we believe DNA-templated silver nanoclusters’ unprecedented unique properties will finally benefit medical progress.

用DNA支架稳定的银纳米簇具有出色的物理和化学特性，已成为生物医学系统中的通用工具。银纳米团簇作为尺寸小于2 nm的后期纳米材料，具有良好的稳定性和强荧光性。通过使用具有不同序列和结构的DNA，可以轻松制备可溶的以DNA为模板的银纳米簇。它们具有可调的荧光发射，并适合多功能设计。已经对该生物相容性材料在生物传感，生物成像和治疗方面的应用潜力进行了广泛的努力。在这里，我们致力于概述生物医学应用中以DNA为模板的银纳米簇的最新进展。然后，考虑到不断发展的进步，讨论了未来的前景和挑战。