New methods to control local RNA methylation are needed to elucidate the function of individual m6A. Here, fusion proteins between the programmable RNA binding protein PUF and the m6A demethylase FTO or METTL14 methyltransferase were designed. FTO-PUFs and METTL14-PUFs showed sequence-specific RNA demethylation and methylation activities, respectively.

中文翻译：

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使用PUF RNA结合蛋白的可编程RNA甲基化和去甲基化。

需要新的控制局部RNA甲基化的方法来阐明单个m6A的功能。在这里，设计了可编程RNA结合蛋白PUF和m6A脱甲基酶FTO或METL14甲基转移酶之间的融合蛋白。FTO-PUF和METTL14-PUF分别显示了序列特异性RNA去甲基化和甲基化活性。