This study aimed to evaluate the infection of the marsupial Monodelphis domestica (the gray short-tailed opossum) by Rickettsia parkeri and its role as an amplifier of the bacterium for Amblyomma ticks. Ten M. domestica males were inoculated with phosphate-buffered saline (PBS) and 10⁶ Vero cells infected with R. parkeri. In seven animals, inoculation was intramuscular, and in three intraperitoneal. One male (control) received 1 ml of the same vehicle used for inoculation intraperitoneally. The three animals inoculated intraperitoneally were infested with uninfected A. sculptum larvae and nymphs between the 2^nd and 9^th day post-infection (DPI). Parasitemia was monitored from the 3^rd to 9^th DPI by polymerase chain reaction, using primers for 17 kDa and ompA. The animals were also clinically evaluated. Of the animals infected intramuscularly, only one was blood-positive by the 5th DPI. The three animals infected intraperitoneally were blood-positive on the 2nd, 5th, 7th, and 9th DPI. Of the ten pools of recovered engorged ticks, six had positive bands. The kidney, liver, heart, and spleen of an intramuscularly infected animal were also positive. The rectal temperature of the animals tested increased only in the first three DPI. The animals inoculated intraperitoneally showed prostration, bristled hair, and weight loss. The study found that R. parkeri was capable of infecting M. domestica, which developed rickettsemia and caused infection in xenodiagnostic ticks.

这项研究旨在评估立克次氏体立克次氏菌对有袋有毛monodelphis domestica（灰色短尾负鼠）的感染及其作为细菌性A虫mb虫的放大器的作用。十家蝇男性用磷酸盐缓冲盐水（PBS）和10个接种⁶感染Vero细胞R. parkeri。在七只动物中，肌内接种，在三只腹膜内。一位男性（对照组）接受了1毫升腹膜内接种的相同媒介物。该三只动物经腹膜内接种用未感染的侵染A. sculptum 2之间的幼虫和蛹^ND和9^次感染后一天（DPI）。寄生虫血症是从3监视^次至9^个通过聚合酶链反应DPI，使用17kDa的和引物的ompA。还对动物进行了临床评估。肌肉内感染的动物中，只有第5个DPI的血液阳性。腹膜内感染的三只动物在DPI的第2、5、7和9日均为血液阳性。在恢复的充血tick的十个池中，六个具有正带。肌肉内感染的动物的肾脏，肝脏，心脏和脾脏也呈阳性。仅在前三个DPI中，被测动物的直肠温度升高。腹膜内接种的动物表现出虚脱，毛发毛发和体重减轻。研究发现，R。parkeri是能够感染的中号。domestica，其发展为立克次体血症，并在异种诊断tick中引起感染。