The aim of the present study was to evaluate the differences in salivary proteome at rest and in response to an acute exercise in men and women. For this, unstimulated whole saliva samples in rest and after a bout resistance exercise leading to failure (ELF) of both men and women (n = 5 for each sex) were subjected to isobaric Tandem Mass Tags (TMT) labelling followed by LC-MS/MS. A total of 274 proteins were identified and met the inclusion criteria. 16 proteins were modulated for the interaction sex*exercise, 6 were modulated because of the exercise, and 65 were differentially expressed between men and women at rest. In conclusion, these results indicate sex-related differences in the salivary proteome at rest and after an acute exercise, pointing out possible candidate biomarkers for sports performance and allowing further knowledge of the physiological processes occurring during ELF. SIGNIFICANCE: The present study describes for the first time the changes that occur in salivary proteome detected by TMT-based proteomics in response to an acute exercise and the differences in these changes depending on sex. Of the 274 protein identified, the 87 differentially expressed proteins and their related pathways were discussed, focusing on the sex- and exercise-related differences in the salivary proteome.

中文翻译：

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男女静息和急性运动后唾液蛋白质组的差异：一项试点研究。

本研究的目的是评估男性和女性在静止和唾液中蛋白质组的差异。为此，对静息状态下和在导致男性和女性失败（ELF）失败（ELF）的男女进行折弯运动后未刺激的全唾液样本进行等压串联质量标签（TMT）标记，然后进行LC-MS /小姐。总共鉴定出274种蛋白质并符合纳入标准。调节了16种蛋白质的相互作用，从而锻炼了性生活，调节了6种蛋白质，这是由于运动引起的，其中65种在休息时男女之间差异表达。总之，这些结果表明静止和急性运动后唾液蛋白质组中与性别相关的差异，指出运动表现的可能候选生物标志物，并允许进一步了解ELF期间发生的生理过程。重要性：本研究首次描述了基于TMT的蛋白质组学在急性运动中唾液蛋白质组中检测到的变化，以及这些变化的差异取决于性别。在鉴定出的274种蛋白质中，讨论了87种差异表达的蛋白质及其相关途径，重点是唾液蛋白质组中与性别和运动有关的差异。