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Identification of genes related to skeletal muscle growth and development by integrated analysis of transcriptome and proteome in myostatin-edited Meishan pigs.
Journal of Proteomics ( IF 2.8 ) Pub Date : 2019-12-24 , DOI: 10.1016/j.jprot.2019.103628 Xiang Li 1 , Shanshan Xie 1 , Lili Qian 1 , Chunbo Cai 1 , Hanfang Bi 1 , Wentao Cui 1
Journal of Proteomics ( IF 2.8 ) Pub Date : 2019-12-24 , DOI: 10.1016/j.jprot.2019.103628 Xiang Li 1 , Shanshan Xie 1 , Lili Qian 1 , Chunbo Cai 1 , Hanfang Bi 1 , Wentao Cui 1
Affiliation
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Embryonic development of skeletal muscle is a complex process that is important to the growth of skeletal muscle after birth. However, the mechanisms by which skeletal muscle growth and development in embryonic phase remain unclear. We have previously produced myostatin-knockout (MKO) Meishan pigs with double-muscle (DM) phenotype via zinc finger nucleases (ZFN) technology. To further investigate the molecular mechanisms involved in skeletal muscle growth and development, in this study, we performed an integrated analysis of transcriptome and proteome in longissimus dorsi muscle from myostatin wild type (MWT) and MKO pigs on 65 days post coitus. Results showed that, compared with MWT group, there were 438 differentially expressed genes (DEGs) and 66 differentially expressed proteins (DEPs) in MKO group. These DEGs and DEPs were mainly enriched in signaling pathways that are involved in skeletal muscle growth and development, glucose metabolism and apoptosis. Furthermore, we identified two key genes, Troponin T 1 (TNNT1) and Myosin regulatory light chain 9 (MYL9), which showed significant changes in both mRNA and protein levels with the similar changing trends in MKO group. It is thus speculated that TNNT1 and MYL9 may play an important role in skeletal muscle growth and development. SIGNIFICANCE: Our study analyzed some important regulatory genes and proteins during skeletal muscle growth and development, our results provided (1) a new insight to further understanding of the molecular mechanisms by which growth and development are regulated in porcine skeletal muscle, and (2) some possible molecular makers for improvement of meat quality in the animal husbandry and diagnosis of human muscle diseases in medicine.
中文翻译：
通过对肌生长抑制素编辑的眉山猪的转录组和蛋白质组进行综合分析，鉴定与骨骼肌生长和发育有关的基因。
骨骼肌的胚胎发育是一个复杂的过程，对出生后骨骼肌的生长很重要。但是，尚不清楚胚胎期骨骼肌生长发育的机制。我们以前通过锌指核酸酶（ZFN）技术生产了具有双肌肉（DM）表型的Myostatin基因敲除（MKO）梅山猪。为了进一步研究参与骨骼肌生长和发育的分子机制，在这项研究中，我们在性交后65天对来自肌肉生长抑制素野生型（MWT）和MKO猪的背最长肌的转录组和蛋白质组进行了综合分析。结果表明，与MWT组相比，MKO组有438个差异表达基因（DEGs）和66个差异表达蛋白（DEPs）。这些DEG和DEP主要富含涉及骨骼肌生长和发育，葡萄糖代谢和细胞凋亡的信号传导途径。此外，我们确定了两个关键基因，肌钙蛋白T 1（TNNT1）和肌球蛋白调节性轻链9（MYL9），它们在mRNA和蛋白质水平上均表现出显着变化，而MKO组的变化趋势相似。因此推测TNNT1和MYL9可能在骨骼肌生长发育中起重要作用。意义：我们的研究分析了骨骼肌生长和发育过程中的一些重要调控基因和蛋白质，我们的结果提供了（1）新见识，可进一步了解猪骨骼肌生长和发育受到调控的分子机制，
更新日期：2019-12-25
中文翻译：
通过对肌生长抑制素编辑的眉山猪的转录组和蛋白质组进行综合分析，鉴定与骨骼肌生长和发育有关的基因。
骨骼肌的胚胎发育是一个复杂的过程，对出生后骨骼肌的生长很重要。但是，尚不清楚胚胎期骨骼肌生长发育的机制。我们以前通过锌指核酸酶（ZFN）技术生产了具有双肌肉（DM）表型的Myostatin基因敲除（MKO）梅山猪。为了进一步研究参与骨骼肌生长和发育的分子机制，在这项研究中，我们在性交后65天对来自肌肉生长抑制素野生型（MWT）和MKO猪的背最长肌的转录组和蛋白质组进行了综合分析。结果表明，与MWT组相比，MKO组有438个差异表达基因（DEGs）和66个差异表达蛋白（DEPs）。这些DEG和DEP主要富含涉及骨骼肌生长和发育，葡萄糖代谢和细胞凋亡的信号传导途径。此外，我们确定了两个关键基因，肌钙蛋白T 1（TNNT1）和肌球蛋白调节性轻链9（MYL9），它们在mRNA和蛋白质水平上均表现出显着变化，而MKO组的变化趋势相似。因此推测TNNT1和MYL9可能在骨骼肌生长发育中起重要作用。意义：我们的研究分析了骨骼肌生长和发育过程中的一些重要调控基因和蛋白质，我们的结果提供了（1）新见识，可进一步了解猪骨骼肌生长和发育受到调控的分子机制，
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