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Cyclic electron flow modulate the Linear electron flow and Reactive oxygen species in tomato leaves under high temperature
Plant Science ( IF 4.2 ) Pub Date : 2020-03-01 , DOI: 10.1016/j.plantsci.2019.110387
Jiazhi Lu 1 , Zepeng Yin 1 , Tao Lu 2 , Xiaolong Yang 1 , Feng Wang 1 , Mingfang Qi 1 , Tianlai Li 1 , Yufeng Liu 1
Affiliation  

The cyclic electron flow (CEF) around photosystem I (PSI) plays a crucial role in photosynthesis and also functions in plant tolerance of abiotic environmental stress. However, the role of PGR5/PGRL1- and NDH-dependent CEF in tomato under hightemperature (HT) is poorly understood. Here, we assessed the photoprotective effect of these pathways in tomato leaves under HT by using antimycin A (AA) and rotenone (R), which are chemical inhibitors of PGR5/PGRL1- and NDH-dependent CEF, respectively. The results showed that AA treatment caused significantly greater inhibition of CEF under HT compared to R treatment. Moreover, AA treatment caused a greater decrease in maximal photochemistry efficiency (Fv/Fm) and increased damage to the donor and acceptor side of photosystem II (PSII); however, the limitation of the acceptor side in PSI [Y(NA)] was significantly increased. In addition, thylakoid membrane integrity was compromised and reactive oxygen species, proton gradient (ΔpH), antioxidant enzyme activity, and the expression of photosystem core subunit genes were significantly decreased under AA treatment. These findings indicate that PGR5/PGRL1-dependent CEF protects PSII and PSI from photooxidative damage through the formation of ΔpH while maintaining thylakoid membrane integrity and normal gene expression levels of core photosystem components. This study demonstrates that PGR5/PGRL1-dependent CEF plays a major role in HT response in tomato.

中文翻译:

循环电子流调节高温下番茄叶片中的线性电子流和活性氧

光系统 I (PSI) 周围的循环电子流 (CEF) 在光合作用中起着至关重要的作用,并且还在植物对非生物环境胁迫的耐受性中起作用。然而,对高温 (HT) 下番茄中 PGR5/PGRL1 和 NDH 依赖性 CEF 的作用知之甚少。在这里,我们通过使用分别是 PGR5/PGRL1 和 NDH 依赖性 CEF 的化学抑制剂的抗霉素 A (AA) 和鱼藤酮 (R) 评估了这些途径在 HT 下番茄叶片中的光保护作用。结果表明,与 R 处理相比,AA 处理在 HT 下对 CEF 产生了显着更大的抑制。此外,AA 处理导致最大光化学效率 (Fv/Fm) 的更大下降,并增加了对光系统 II (PSII) 供体和受体侧的损伤;然而,PSI [Y(NA)] 中受体侧的限制显着增加。此外,在AA处理下,类囊体膜完整性受到损害,活性氧、质子梯度(ΔpH)、抗氧化酶活性和光系统核心亚基基因的表达显着降低。这些发现表明 PGR5/PGRL1 依赖性 CEF 通过形成 ΔpH 保护 PSII 和 PSI 免受光氧化损伤,同时保持类囊体膜完整性和核心光系统组件的正常基因表达水平。该研究表明 PGR5/PGRL1 依赖性 CEF 在番茄的 HT 反应中起主要作用。AA处理显着降低光系统核心亚基基因的表达。这些发现表明 PGR5/PGRL1 依赖性 CEF 通过形成 ΔpH 保护 PSII 和 PSI 免受光氧化损伤,同时保持类囊体膜完整性和核心光系统组件的正常基因表达水平。该研究表明 PGR5/PGRL1 依赖性 CEF 在番茄的 HT 反应中起主要作用。AA处理显着降低光系统核心亚基基因的表达。这些发现表明 PGR5/PGRL1 依赖性 CEF 通过形成 ΔpH 保护 PSII 和 PSI 免受光氧化损伤,同时保持类囊体膜完整性和核心光系统组件的正常基因表达水平。该研究表明 PGR5/PGRL1 依赖性 CEF 在番茄的 HT 反应中起主要作用。
更新日期:2020-03-01
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