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Scrophularia buergeriana attenuates allergic inflammation by reducing NF-κB activation.
Phytomedicine ( IF 6.7 ) Pub Date : 2019-12-24 , DOI: 10.1016/j.phymed.2019.153159 Na-Rae Shin 1 , A Yeong Lee 2 , Jun-Ho Song 2 , Sungyu Yang 2 , Inkyu Park 2 , Je-Oh Lim 1 , Tae-Yang Jung 1 , Je-Won Ko 1 , Jong-Choon Kim 1 , Kyung Seob Lim 3 , Min Young Lee 4 , In-Sik Shin 1 , Joong Sun Kim 2
Phytomedicine ( IF 6.7 ) Pub Date : 2019-12-24 , DOI: 10.1016/j.phymed.2019.153159 Na-Rae Shin 1 , A Yeong Lee 2 , Jun-Ho Song 2 , Sungyu Yang 2 , Inkyu Park 2 , Je-Oh Lim 1 , Tae-Yang Jung 1 , Je-Won Ko 1 , Jong-Choon Kim 1 , Kyung Seob Lim 3 , Min Young Lee 4 , In-Sik Shin 1 , Joong Sun Kim 2
Affiliation
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BACKGROUND
Scrophularia buergeriana Miq. (Scrophulariaceae) (SB) has been used as an oriental medicine for the treatment of inflammatory diseases, such as neuritis and pharyngolaryngitis.
PURPOSE
We explored the therapeutic effects of S. buergeriana ethanol extract (SBE) on airway inflammation in ovalbumin (OVA)-induced asthmatic mice and lipopolysaccharide (LPS)-stimulated RAW264.7 cells.
METHODS
Mice were intraperitoneally injected with OVA on days 0 and 14 to elevate the immune response. On days 21 to 23, the mice were challenged with OVA solution and SBE (20 and 40 mg/kg) was administered daily by oral gavage from days 18 to 23. RAW264.7 cells were pretreated with SBE 1 h before LPS stimulation.
RESULTS
SBE administration effectively suppressed inflammatory cell infiltration, the expression of interleukin (IL)-5, IL-13, and IL-17, immunoglobulin E, and airway hyperresponsiveness in an OVA-induced allergic asthma model. A reduction in histological alterations, including airway inflammation and mucus hypersecretion, was observed. These effects of SBE were accompanied by a decrease in matrix metalloproteinase-9 (MMP-9) expression and nuclear factor kappa B (NF-κB) phosphorylation. These responses were observed in LPS-stimulated RAW264.7 cells. SBE treatment reduced the mRNA expression of tumor necrosis factor (TNF)-α, IL-6, and MMP-9, and NF-κB phosphorylation, in LPS-stimulated RAW264.7 cells.
CONCLUSION
Our results indicated that SBE effectively attenuated airway inflammation in an OVA-induced allergic asthma model. These properties of SBE were thought to be involved in the suppression of NF-κB phosphorylation, suggesting that the material has the potential to regulate the development of allergic asthma.
中文翻译：
玄参（Scrophularia buergeriana）通过减少NF-κB的活化来减轻过敏性炎症。
背景技术玄参（Scrophularia buergeriana Miq）。（玄参科）（SB）已被用作治疗诸如神经炎和咽喉炎的炎性疾病的东方药物。目的我们探索了布尔氏链球菌乙醇提取物（SBE）对卵清蛋白（OVA）诱导的哮喘小鼠和脂多糖（LPS）刺激的RAW264.7细胞的气道炎症的治疗作用。方法在第0天和第14天给小鼠腹膜内注射OVA以增强免疫应答。在第21至23天，用OVA溶液攻击小鼠，并从第18至23天每天通过管饲法每天给予SBE（20和40 mg / kg）。在LPS刺激之前1小时，用SBE预处理RAW264.7细胞。结果SBE给药可有效抑制炎症细胞浸润，白介素（IL）-5，IL-13和IL-17的表达，免疫球蛋白E和OVA诱发的过敏性哮喘模型中的气道高反应性。观察到组织学改变的减少，包括气道炎症和粘液分泌过多。SBE的这些作用伴随着基质金属蛋白酶9（MMP-9）表达的减少和核因子κB（NF-κB）磷酸化的降低。这些反应在LPS刺激的RAW264.7细胞中观察到。SBE处理在LPS刺激的RAW264.7细胞中降低了肿瘤坏死因子（TNF）-α，IL-6和MMP-9的mRNA表达以及NF-κB磷酸化。结论我们的结果表明SBE在OVA诱发的过敏性哮喘模型中有效减轻了气道炎症。SBE的这些特性被认为与NF-κB磷酸化的抑制有关，
更新日期：2019-12-25
中文翻译：
玄参（Scrophularia buergeriana）通过减少NF-κB的活化来减轻过敏性炎症。
背景技术玄参（Scrophularia buergeriana Miq）。（玄参科）（SB）已被用作治疗诸如神经炎和咽喉炎的炎性疾病的东方药物。目的我们探索了布尔氏链球菌乙醇提取物（SBE）对卵清蛋白（OVA）诱导的哮喘小鼠和脂多糖（LPS）刺激的RAW264.7细胞的气道炎症的治疗作用。方法在第0天和第14天给小鼠腹膜内注射OVA以增强免疫应答。在第21至23天，用OVA溶液攻击小鼠，并从第18至23天每天通过管饲法每天给予SBE（20和40 mg / kg）。在LPS刺激之前1小时，用SBE预处理RAW264.7细胞。结果SBE给药可有效抑制炎症细胞浸润，白介素（IL）-5，IL-13和IL-17的表达，免疫球蛋白E和OVA诱发的过敏性哮喘模型中的气道高反应性。观察到组织学改变的减少，包括气道炎症和粘液分泌过多。SBE的这些作用伴随着基质金属蛋白酶9（MMP-9）表达的减少和核因子κB（NF-κB）磷酸化的降低。这些反应在LPS刺激的RAW264.7细胞中观察到。SBE处理在LPS刺激的RAW264.7细胞中降低了肿瘤坏死因子（TNF）-α，IL-6和MMP-9的mRNA表达以及NF-κB磷酸化。结论我们的结果表明SBE在OVA诱发的过敏性哮喘模型中有效减轻了气道炎症。SBE的这些特性被认为与NF-κB磷酸化的抑制有关，
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