Successful implantation happens only when the development of a competent blastocyst synchronized with the differentiation of a receptive uterus. The exact mechanism affecting embryo implantation competency is still unclear. Previous data from our laboratory showed that several members of the let-7 family were up-regulated in the implanting dormant blastocysts and prohibited embryo activation through down-regulation integrin-β3. However, how the mir-let-7 family is regulated is still a question. In this study, the in vitro co-culture model was applied to imitate implantation. Human embryo surrogate Jeg-3 spheroids and endometrium epithelial cells Ishikawa were used. The following views were demonstrated. Firstly，Wnt/β-catenin signaling is essential for Jeg-3 spheroids implantation. Secondly, mir-let-7a is repressed by Wnt signaling, and low let-7a is beneficial for spheroids attachment and outgrowth. Third, in contrast with let-7a, lin28a is up-regulated by Wnt and promotes attachment and outgrowth. Lastly, the function of Wnt in embryo surrogate spheroids in implantation is mediated through lin28a/let-7a axis. In summary, our findings suggest Wnt/β-catenin signaling strength human embryo surrogate spheroids implanting potential through regulation lin28a/let-7a axis.

中文翻译：

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通过Wnt /β-catenin途径和lin28a / let-7a轴调节人滋养细胞替代Jeg-3球体的植入潜力。

仅当感受态胚泡的发育与接受性子宫的分化同步进行时，成功植入才发生。影响胚胎植入能力的确切机制仍不清楚。来自我们实验室的先前数据表明，let-7家族的几个成员在植入休眠的胚泡中被上调，并通过下调整联蛋白-β3抑制了胚胎的激活。但是，mir-let-7家族如何受到监管仍是一个问题。在这项研究中，体外共培养模型被应用于模仿植入。使用人胚替代Jeg-3球体和子宫内膜上皮细胞Ishikawa。演示了以下视图。首先，Wnt /β-catenin信号传导对于Jeg-3球体植入至关重要。其次，mir-let-7a被Wnt信号抑制，低let-7a有利于球体的附着和生长。第三，与let-7a相反，lin28a被Wnt上调并促进附着和生长。最后，Wnt在胚胎代孕球体植入过程中的功能是通过lin28a / let-7a轴介导的。总而言之，我们的研究结果表明Wnt /β-catenin信号强度可以通过调节lin28a / let-7a轴替代人胚的类球体植入潜能。