Protein adsorption on biomaterial surfaces has been investigated in the development of protein-repellent implantable devices. While the study of the adsorption of a single protein have produced valuable insights of the role of specific proteins in the biological responses to biomaterials, a systematic high throughput screening method is needed to gain more comprehensive understanding of such a complex process, mainly because biomaterials are exposed to protein mixtures when implanted in the human body. To further advance our knowledge of the dynamics of protein adsorption/desorption at interfaces between proteins and solid surfaces, proteomic technologies have been explored to determine relationships between adsorbed proteins on the surfaces and subsequent biological responses. In this review, we will briefly describe the protein adsorption process and proteomics technologies and focus on subsequent biological responses to biomaterials such as blood/biomaterial interactions, biocompatibility, and cell behavior, to obtain more comprehensive understanding of the process for the development of improved biomaterials. We also highlight a number of challenges of contemporary proteomics technologies and future perspectives to advance our knowledge of protein adsorption/desorption dynamics on the surfaces of biomaterials.

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使用蛋白质组学表征蛋白质在生物材料表面吸附动力学的系统方法。

在开发可排斥蛋白质的植入设备中，已经研究了蛋白质在生物材料表面上的吸附。虽然对单个蛋白质吸附的研究已对特定蛋白质在对生物材料的生物反应中的作用产生了有价值的见解，但仍需要系统的高通量筛选方法来更全面地了解这种复杂的过程，这主要是因为生物材料是植入人体后会暴露于蛋白质混合物中。为了进一步提高我们对蛋白质与固体表面之间界面处蛋白质吸附/解吸动力学的认识，已经研究了蛋白质组学技术，以确定表面上吸附的蛋白质与后续生物学反应之间的关系。在这篇评论中，我们将简要描述蛋白质吸附过程和蛋白质组学技术，并将重点关注对生物材料的后续生物反应，例如血液/生物材料的相互作用，生物相容性和细胞行为，以获得对开发改进的生物材料的过程的更全面的了解。我们还强调了当代蛋白质组学技术和未来观点的许多挑战，以提高我们对生物材料表面蛋白质吸附/解吸动力学的认识。