The complexity of experimental schemes still challenges the broad application of multimodal microscopy beyond fundamental research. Pulse shaping has been a promising candidate to assist in the implementation of flexible and compact schemes. In this work, its application is demonstrated for vibrational resonant sum frequency (SF) spectroscopy in a nonlinear Raman microscope. The mixing of an ultrabroadband NIR laser pulse with a difference-frequency-generated MIR spectrum provides spectral access from ${1750}\,\,{{\rm cm}^{ - 1}}$ to beyond ${3000}\,\,{{\rm cm}^{ - 1}}$ via two shaping methods: (i) homodyne MIR scanning and (ii) heterodyne multiplex. The versatility of shaper-based SF is demonstrated in the spectroscopy and microscopy of crystalline cholesterol and cysteine.

中文翻译：

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灵活的脉冲整形，适用于和频显微光谱

除了基础研究之外，实验方案的复杂性仍然挑战着多峰显微镜的广泛应用。脉冲整形已成为有希望的候选者，以协助实施灵活而紧凑的方案。在这项工作中，证明了其在非线性拉曼显微镜中用于振动共振和频率（SF）光谱的应用。将超宽带NIR激光脉冲与差频生成的MIR频谱混合，可提供从$ {1750} \，\，{{\ rm cm} ^ {-1}} $到$ {3000} \以上的频谱访问权限， \，{{\ rm cm} ^ {-1}} $通过两种整形方法：（i）零差MIR扫描和（ii）外差多路复用。在结晶胆固醇和半胱氨酸的光谱和显微镜检查中证明了基于成形剂的SF的多功能性。