The aim of this work was to develop a novel fully natural drug delivery system for the treatment of acne, based on core-shell microcapsules that contain Berberine (Brb). The two main objectives of the work were: a) the synthesis and the characterization of complex microcapsules (ms), ms encapsulating Berberine (ms-Brb), and b) in vitro evaluation of the release of Brb, of the cytotoxicity on normal skin cells and of the antimicrobial effect on Cutibacterium acnes (formerly Propionibacterium acnes) (C. acnes). For a), bovine serum albumin (BSA) gel-core microcapsules with alternating multilayer shells of calcium cross-linked Pectin (P) hydrogel and the polyelectrolyte complex formed by P and Chitosan (Chi) (BSA gel/P/Chi/P) were synthesized. The BSA gel-core microcapsules were obtained using a sacrificial CaCO₃ template method, while the multilayer shell was formed through a technique consisting in the layer-by-layer (Lbl) deposition of polyelectrolyte complex formed by P and Chi. Brb was encapsulated into the resulting microcapsules, by a process of diffusion from solution. The structure characterization of ms/ms-Brb was performed by FTIR and UV–Vis spectroscopy, X-ray diffraction, confocal laser scanning microscopy, and scanning electron microscopy. The in vitro assessment of ms/ms-Brb cytotoxicity on skin cells was performed using keratinocyte (HaCaT) cell line. Results of physicochemical analyses confirm the successful encapsulation of Brb in ms, and the in vitro biological study recommends ms-Brb as a candidate for future in vivo research targeting anti-acne treatment.

这项工作的目的是基于包含小ber碱（Brb）的核壳微胶囊，开发一种新型的完全天然的用于治疗痤疮的药物输送系统。这项工作的两个主要目标是：a）合成和表征复杂的微囊（ms），包封小ber碱的ms（ms-Brb），以及b）体外评估Brb的释放，对正常皮肤的细胞毒性细胞和对痤疮杆菌（以前的丙酸丙酸杆菌）（痤疮丙酸杆菌）有抗菌作用。）。对于a），牛血清白蛋白（BSA）凝胶核心微胶囊具有交替交联的钙交联的果胶（P）水凝胶多层壳和由P和壳聚糖（Chi）形成的聚电解质复合物（BSA gel / P / Chi / P）被合成。使用牺牲CaCO ₃模板法获得BSA凝胶核心微囊，而多层壳则通过包括逐层（Lbl）沉积由P和Chi形成的聚电解质复合物的技术来形成。通过从溶液中扩散的过程，将Brb包封到所得的微囊中。通过FTIR和UV-Vis光谱，X射线衍射，共聚焦激光扫描显微镜和扫描电子显微镜对ms / ms-Brb进行结构表征。在体外使用角质形成细胞（HaCaT）细胞系对ms / ms-Brb对皮肤细胞的细胞毒性进行评估。物理化学分析的结果证实了Brb在ms中的成功包封，并且体外生物学研究推荐ms-Brb作为未来针对抗痤疮治疗的体内研究的候选药物。