The generation of genomically stable and functional oocytes has great potential for preserving fertility and restoring ovarian function. It remains elusive whether functional oocytes can be generated from adult female somatic cells through reprogramming to germline-competent pluripotent stem cells (gPSCs) by chemical treatment alone. Here, we show that somatic granulosa cells isolated from adult mouse ovaries can be robustly induced to generate gPSCs by a purely chemical approach, with additional Rock inhibition and critical reprogramming facilitated by crotonic sodium or acid. These gPSCs acquired high germline competency and could consistently be directed to differentiate into primordial-germ-cell-like cells and form functional oocytes that produce fertile mice. Moreover, gPSCs promoted by crotonylation and the derived germ cells exhibited longer telomeres and high genomic stability like PGCs in vivo, providing additional evidence supporting the safety and effectiveness of chemical induction, which is particularly important for germ cells in genetic inheritance.

中文翻译：

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来源于颗粒细胞的功能性卵母细胞。

基因组稳定和功能正常的卵母细胞的产生具有保持生育力和恢复卵巢功能的巨大潜力。仅通过化学处理就可以将成年女性的体细胞通过重编程为能适应生殖细胞的多能干细胞（gPSC）来产生功能性卵母细胞，这一点仍然难以捉摸。在这里，我们表明，从成年小鼠卵巢分离的体粒细胞可以通过纯化学方法强烈诱导生成gPSC，同时巴豆酸或巴豆酸可以促进其他岩石抑制和关键性重编程。这些gPSC具有很高的种系能力，可以始终被定向分化为原始生殖细胞样细胞，并形成能繁殖小鼠的功能性卵母细胞。而且，