当前位置: X-MOL 学术Ultrason. Sonochem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Effect of the lactose source on the ultrasound-assisted enzymatic production of galactooligosaccharides and gluconic acid.
Ultrasonics Sonochemistry ( IF 8.7 ) Pub Date : 2019-12-23 , DOI: 10.1016/j.ultsonch.2019.104945
Fabián Rico-Rodríguez 1 , Mar Villamiel 2 , Laura Ruiz-Aceituno 2 , Juan Carlos Serrato 1 , Antonia Montilla 2
Affiliation  

It is well known that one of the main problems in galactooligosaccharide production (GOS) via tranglycosylation of lactose is the presence of monosaccharides that contribute to increasing the glycaemic index, as is the case of glucose. In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey). In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor. The highest yields of GOS production with the lowest glucose concentration and highest GA production were obtained with lactose solution in multienzymatic systems in the presence of ultrasound (30% amplitude) when Gox was added after 1 h of treatment with β-gal. This work demonstrates the ability of US to enhance efficiently the obtainment of prebiotic mixtures of low glycaemic index.

中文翻译:

乳糖源对低聚半乳糖和葡萄糖酸的超声辅助酶促生产的影响。

众所周知,通过乳糖的转糖基化生产低聚半乳糖(GOS)的主要问题之一是存在单糖,其有助于增加血糖指数,如葡萄糖的情况。在这项工作中,以及研究超声波(US)对葡萄糖酸(GA)生产过程中葡萄糖氧化酶(Gox)活化的影响,我们已经进行了对多酶反应中葡萄糖选择性氧化为葡萄糖酸的研究(β使用不同来源的乳糖作为底物(乳糖溶液,乳清渗透液,干酪乳清),在Power US的协助下辅助使用β-半乳糖苷酶(β-gal)和Gox。就基质对GOS和GA产生的影响而言,乳糖溶液效果最好,其次是干酪乳清和渗透乳清,盐成分是影响最大的因素。当在β-gal处理1小时后加入Gox时,在超声存在下(30%振幅),在多酶系统中用乳糖溶液获得具有最低葡萄糖浓度和最高GA产量的最高GOS产量。这项工作证明了美国有效提高低血糖指数的益生元混合物的能力。
更新日期:2019-12-23
down
wechat
bug