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A novel fluorescent probe based on naphthalimide for imaging nitroreductase (NTR) in bacteria and cells.
Bioorganic & Medicinal Chemistry ( IF 3.3 ) Pub Date : 2019-12-24 , DOI: 10.1016/j.bmc.2019.115280
Zijian Zhang 1 , Ting Lv 1 , Binbin Tao 2 , Zhenfu Wen 1 , Yongqian Xu 1 , Hongjuan Li 1 , Fengyu Liu 3 , Shiguo Sun 1
Affiliation  

A nitroreductase (NTR) responsive fluorescent probe, Na-NO2, comprising p-nitrobenzyl as the unique recognition group and 1,8-naphthalimide as fluorophore, was synthesized. Na-NO2 showed remarkable fluorescence "turn-on" signal in the presence of NTR under DMSO/H2O (1:19, v/v) buffered with PBS (pH = 7) solution in the presence of NADH (300 µM). Furthermore, the probe has a low detection limit down to 3.4 ng/mL and it is very sensitive towards the NTR in Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), normal and tumor cells such as HL-7702, HepG-2 and MCF-7.

中文翻译:

一种新型的基于萘二甲酰亚胺的荧光探针,可对细菌和细胞中的硝基还原酶(NTR)进行成像。

合成了一种对硝基还原酶(NTR)响应的荧光探针Na-NO2,该探针包含对硝基苄基作为唯一识别基团和1,8-萘二甲酰亚胺作为荧光团。Na-NO2在NTR存在下,在DMSO / H2O(1:19,v / v)和PBS(pH = 7)缓冲液,在NADH(300 µM)存在的情况下,在NTR存在下显示出显着的荧光“开启”信号。此外,该探针的检测限低至3.4 ng / mL,并且对大肠杆菌(E. coli),金黄色葡萄球菌(S. aureus),正常细胞和肿瘤细胞(例如HL-7702, HepG-2和MCF-7。
更新日期:2019-12-25
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