BACKGROUND With the increasing amount of high-throughput genomic sequencing data, there is a growing demand for a robust and flexible tool to perform interaction analysis. The identification of SNP-SNP, SNP-CpG, and higher order interactions helps explain the genetic etiology of human diseases, yet genome-wide analysis for interactions has been very challenging, due to the computational burden and a lack of statistical power in most datasets. RESULTS The wtest R package performs association testing for main effects, pairwise and high order interactions in genome-wide association study data, and cis-regulation of SNP and CpG sites in genome-wide and epigenome-wide data. The software includes a number of post-test diagnostic and analysis functions and offers an integrated toolset for genetic epistasis testing. CONCLUSIONS The wtest is an efficient and powerful statistical tool for integrated genetic epistasis testing. The package is available in CRAN: https://CRAN.R-project.org/package=wtest.

中文翻译：

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wtest：用于基因上位性测试的集成R软件包。

背景技术随着高通量基因组测序数据数量的增加，对进行交互分析的鲁棒且灵活的工具的需求不断增长。SNP-SNP，SNP-CpG和高阶相互作用的鉴定有助于解释人类疾病的遗传病因，但是由于大多数数据集中的计算负担和缺乏统计能力，因此全基因组相互作用的分析一直非常具有挑战性。结果wtest R软件包对全基因组关联研究数据中的主要作用，成对和高阶相互作用以及对全基因组和表观基因组数据中SNP和CpG位点的顺式调节进行关联测试。该软件包括大量的测试后诊断和分析功能，并提供了用于基因上位性测试的集成工具集。结论wtest是用于综合遗传上位测试的有效而强大的统计工具。该软件包可在CRAN中找到：https：//CRAN.R-project.org/package=wtest。