CRISPR-edited variants at the 3'-end of OsLOGL5's coding sequence (CDS), significantly increased rice grain yield under well-watered, drought, normal nitrogen, and low nitrogen field conditions at multiple geographical locations. Cytokinins impact numerous aspects of plant growth and development. This study reports that constitutive ectopic overexpression of a rice cytokinin-activation enzyme-like gene, OsLOGL5, significantly reduced primary root growth, tiller number, and yield. Conversely, mutations at the 3'-end of OsLOGL5 CDS resulted in normal rice plant morphology but with increased grain yield under well-watered, drought, normal nitrogen, and low nitrogen field conditions at multiple geographical locations. Six gene edited variants (Edit A to F) were created and tested in the field. Edit-B and Edit-F plants increased, but Edit-D and Edit-E plants decreased, the panicle number per plant. All OsLOGL5-edited plants significantly increased seed setting rate, total grain numbers, full-filled grain numbers per panicle, and thousand seed weight under drought conditions, suggesting that OsLOGL5 is likely involved in the regulation of both seed development and grain filling processes. Our results indicate that the C-terminal end of OsLOGL5 protein plays an important role in regulating rice yield improvement under different abiotic stress conditions, and OsLOGL5 is important for rice yield enhancement and stability.

中文翻译：

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细胞分裂素激活酶样基因可提高水稻在各种田间条件下的产量。

OsLOGL5 编码序列 (CDS) 3' 末端的 CRISPR 编辑变体在多个地理位置的水分充足、干旱、正常氮和低氮田间条件下显着提高了水稻产量。细胞分裂素影响植物生长和发育的许多方面。本研究报告称，水稻细胞分裂素激活酶样基因 OsLOGL5 的组成型异位过表达显着降低了初生根的生长、分蘖数和产量。相反，OsLOGL5 CDS 3'-末端的突变导致水稻植株形态正常，但在多个地理位置的水分充足、干旱、正常氮和低氮田间条件下，水稻产量增加。在现场创建并测试了六个基因编辑变体（编辑 A 到 F）。Edit-B 和 Edit-F 植物增加，但Edit-D和Edit-E植株减少，单株穗数减少。在干旱条件下，所有经 OsLOGL5 编辑的植物均显着提高了结实率、总粒数、每穗饱满粒数和千粒重，这表明 OsLOGL5 可能参与了种子发育和灌浆过程的调​​控。我们的研究结果表明，OsLOGL5蛋白的C末端在不同非生物胁迫条件下对水稻产量的提高具有重要的调控作用，而OsLOGL5对水稻产量的提高和稳定性也很重要。表明 OsLOGL5 可能参与种子发育和籽粒灌浆过程的调​​节。我们的研究结果表明，OsLOGL5蛋白的C末端在不同非生物胁迫条件下对水稻产量的提高具有重要的调控作用，而OsLOGL5对水稻产量的提高和稳定性也很重要。表明 OsLOGL5 可能参与种子发育和籽粒灌浆过程的调​​节。我们的研究结果表明，OsLOGL5蛋白的C末端在不同非生物胁迫条件下对水稻产量的提高具有重要的调控作用，而OsLOGL5对水稻产量的提高和稳定性也很重要。