A new colorimetric and fluorescent probe MNTPZ based on 1H-imidazo[4,5-b]phenazine derivative has been designed and synthesized for successive detection of Ag+ and I- . The probe MNTPZ shows selective colorimetric response by a change in color from yellow to orange and "turn-off" fluorometric response upon binding with Ag+ in DMSO: Water (pH = 7, 1:1, v/v) over other cations. The binding mode of probe MNTPZ to Ag+ was studied by Job's plot, 1 H NMR studies, FT-IR spectroscopy and DFT calculations. Moreover, the situ generated probe MNTPZ+ Ag+ complex acted as an efficient fluorometric "turn-on" probe for I- via Ag+ displacement approach. The detection limit of probe MNTPZ for Ag+ and the resultant complex probe MNTPZ+ Ag+ for I- were determined to be 1.36 μmol/L and 1.03 μmol/L respectively. Notably, the developed probe was successfully used for quantitative determination of I- in real samples with satisfactory results.

中文翻译：

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基于吩嗪的比色和荧光化学传感器，用于顺序检测水性介质中的Ag +和I-。

设计并合成了一种新的基于1H-咪唑并[4,5-b]吩嗪衍生物的比色荧光探针MNTPZ，用于Ag +和I-的连续检测。探针MNTPZ通过颜色从黄色变为橙色显示选择性的比色响应，并且在与DMSO：水（pH = 7、1：1，v / v）中的Ag +结合后，会出现“关闭”的荧光响应，而不是其他阳离子。探针MNTPZ与Ag +的结合模式通过Job's图，1 H NMR研究，FT-IR光谱和DFT计算进行了研究。此外，原位产生的探针MNTPZ + Ag +络合物通过Ag +置换方法充当了I-的高效荧光“开启”探针。测定的Ag +探针MNTPZ的检出限和I-的复合探针MNTPZ + Ag +的检出限分别为1.36μmol/ L和1.03μmol/ L。尤其，