Mass spectrometry-based proteomics enables the unbiased and sensitive profiling of cellular proteomes and extracellular environments. Recent technological and bioinformatic advances permit identifying dual biological systems in a single experiment, supporting investigation of infection from both the host and pathogen perspectives. At the ocular surface, Pseudomonas aeruginosa is commonly associated with biofilm formation and inflammation of the ocular tissues, causing damage to the eye. The interaction between P. aeruginosa and the immune system at the site of infection describes limitations in clearance of infection and enhanced pathogenesis. Here, the extracellular environment (eye wash) of murine ocular surfaces infected with a clinical isolate of P. aeruginosa is profiled and neutrophil marker proteins are detected, indicating neutrophil recruitment to the site of infection. The first potential diagnostic markers of P. aeruginosa-associated keratitis are also identified. In addition, the deepest murine corneal proteome to date is defined and proteins, categories, and networks critical to the host response are detected. Moreover, the first identification of bacterial proteins attached to the ocular surface is reported. The findings are validated through in silico comparisons and enzymatic profiling. Overall, the work provides comprehensive profiling of the host-pathogen interface and uncovers differences between general and site-specific host responses to infection.

中文翻译：

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无标记的定量蛋白质组学可区分对眼表铜绿假单胞菌感染的一般和特定地点宿主反应。

基于质谱的蛋白质组学可以对细胞蛋白质组和细胞外环境进行无偏和敏感的分析。最新的技术和生物信息学进展允许在单个实验中鉴定双重生物系统，从而支持从宿主和病原体的角度研究感染。在眼表，铜绿假单胞菌通常与生物膜的形成和眼组织的炎症有关，对眼睛造成损害。铜绿假单胞菌与感染部位免疫系统之间的相互作用描述了感染清除和发病机理增强的局限性。在此，对感染了铜绿假单胞菌临床分离株的鼠眼表面的细胞外环境（洗眼液）进行了分析，并检测了中性粒细胞标志物蛋白，表明嗜中性白细胞募集到感染部位。还确定了铜绿假单胞菌相关性角膜炎的第一个潜在诊断标志物。另外，定义了迄今为止最深的鼠角膜蛋白质组，并检测了对宿主反应至关重要的蛋白质，类别和网络。此外，报道了对附着在眼表面的细菌蛋白质的首次鉴定。通过计算机比较和酶谱分析验证了该发现。总体而言，这项工作对宿主-病原体界面进行了全面的分析，并揭示了一般宿主感染和特定于宿主感染的宿主反应之间的差异。定义了迄今为止最深的小鼠角膜蛋白质组，并检测了对宿主反应至关重要的蛋白质，类别和网络。此外，报道了对附着在眼表面的细菌蛋白质的首次鉴定。通过计算机比较和酶谱分析验证了该发现。总体而言，这项工作对宿主-病原体界面进行了全面的分析，并揭示了一般宿主感染和特定于宿主感染的宿主反应之间的差异。定义了迄今为止最深的小鼠角膜蛋白质组，并检测了对宿主反应至关重要的蛋白质，类别和网络。此外，报道了对附着在眼表面的细菌蛋白质的首次鉴定。通过计算机比较和酶谱分析验证了该发现。总体而言，这项工作对宿主-病原体界面进行了全面的分析，并揭示了一般宿主感染和特定于宿主感染的宿主反应之间的差异。