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Morphological and initial molecular characterization of Clogmia albipunctatus larvae (Diptera: Psychodidae) causing urinary myiasis in Egypt.
PLOS Neglected Tropical Diseases ( IF 3.4 ) Pub Date : 2019-12-23 , DOI: 10.1371/journal.pntd.0007887 Haiam Mohammed Mahmoud Farrag 1 , Enas Abdelhameed Mahmoud Huseein 1 , Amal M Almatary 1 , Ragaa A Othman 1
PLOS Neglected Tropical Diseases ( IF 3.4 ) Pub Date : 2019-12-23 , DOI: 10.1371/journal.pntd.0007887 Haiam Mohammed Mahmoud Farrag 1 , Enas Abdelhameed Mahmoud Huseein 1 , Amal M Almatary 1 , Ragaa A Othman 1
Affiliation
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Myiasis is the infestation of human tissues by dipterous fly larvae of the class Insecta. Clogmia albipunctatus, family Psychodidae, is one of the most medically important insects that cause human myiasis. The aim of the present study is the morphological identification and the molecular characterization of moth flies causing many cases of urinary myiasis in Egypt, based on sequencing of the mitochondrial DNA of the larvae. Seven urinary samples of patients complaining of urinary symptoms and giving a history of low socioeconomic level were examined. Recovered larvae were identified using light microscopy and SEM. For molecular identification, the mitochondrial genes Cytochrome B (cytB), NADH1, NADH1, and 16S were sequenced and phylogenetically analyzed. The morphological and molecular characterization could accurately diagnose our patients to have C. albipunctatus infestation. Such results provided the initial set of data on the molecular identification and phylogenetic analysis of moth flies based on DNA barcoding in Egypt.
中文翻译：
在埃及引起尿my虫病的Clogmia albipunctatus幼虫（双翅目：Psychodidae）的形态和初始分子特征。
肌病是通过昆虫纲昆虫的双翅fly幼虫侵染人体组织。衣原体科Clobmia albipunctatus是引起人类肌病的医学上最重要的昆虫之一。本研究的目的是基于幼虫线粒体DNA的测序，对引起埃及许多泌尿系肌病的蛾类进行形态学鉴定和分子表征。检查了七个抱怨尿路症状并具有低社会经济水平病史的患者的尿液样本。使用光学显微镜和SEM鉴定回收的幼虫。为了进行分子鉴定，对线粒体基因细胞色素B（cytB），NADH1，NADH1和16S进行了测序并进行了系统发育分析。形态学和分子特征可以准确地诊断出我们的患者患有白喉衣原体。这些结果为埃及基于DNA条形码的蛾类的分子鉴定和系统发育分析提供了初步的数据。
更新日期：2019-12-25
中文翻译：
在埃及引起尿my虫病的Clogmia albipunctatus幼虫（双翅目：Psychodidae）的形态和初始分子特征。
肌病是通过昆虫纲昆虫的双翅fly幼虫侵染人体组织。衣原体科Clobmia albipunctatus是引起人类肌病的医学上最重要的昆虫之一。本研究的目的是基于幼虫线粒体DNA的测序，对引起埃及许多泌尿系肌病的蛾类进行形态学鉴定和分子表征。检查了七个抱怨尿路症状并具有低社会经济水平病史的患者的尿液样本。使用光学显微镜和SEM鉴定回收的幼虫。为了进行分子鉴定，对线粒体基因细胞色素B（cytB），NADH1，NADH1和16S进行了测序并进行了系统发育分析。形态学和分子特征可以准确地诊断出我们的患者患有白喉衣原体。这些结果为埃及基于DNA条形码的蛾类的分子鉴定和系统发育分析提供了初步的数据。
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