Fecal Metabolomics as a Novel Noninvasive Method for Short-Term Stress Monitoring in Beef Cattle.,Journal of Proteome Research

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Fecal Metabolomics as a Novel Noninvasive Method for Short-Term Stress Monitoring in Beef Cattle.
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2019-12-24 , DOI: 10.1021/acs.jproteome.9b00655
Azzurra Valerio ₁ , Luca Casadei ₁ , Alessandro Giuliani ₂ , Mariacristina Valerio ₁

Affiliation

Traditional measures of short-term stress response such as fecal glucocorticoid metabolites (FGM) are widely used in controlled settings to quantify the intensity of stimulation to which cattle are exposed. However, FGMs present several methodological and interpretation pitfalls when applied on animals in free-ranging conditions. In this study, we proposed an NMR-based fecal metabolomics strategy for noninvasive stress detection in beef cattle. Using a longitudinal sample collection, we monitored the changes in the fecal metabolome and FGM concentrations before and after an acute stressful event. Our results showed that while the fecal metabolome changed as a function of stress (p < 0.001), the mean concentrations of FGM did not change (Levene's test: F-ratio: 0.87, p-value: 0.44). Furthermore, we showed that the interanimal variability observed in the stress response was correlated with the individual fecal microbiota. This result was in line with recent findings, indicating the gut microbiome as a crucial mediator of stress response. We conclude that NMR-based fecal metabolomics proved to be a reliable methodology to assess stress response and that its future applicability to studies for stress monitoring in range animals may be more appropriate than FGM analysis.

中文翻译：

粪便代谢组学是一种用于牛肉牛短期压力监测的新型无创方法。

传统的短期应激反应测量方法，例如粪便糖皮质激素代谢物（FGM），在受控环境中被广泛使用，以量化牛所受到刺激的强度。然而，在自由放养的条件下，外阴残割在动物身上表现出一些方法上的和解释上的陷阱。在这项研究中，我们提出了一种基于NMR的粪便代谢组学策略，用于肉牛的无创压力检测。使用纵向样本采集，我们监测了急性应激事件前后大便代谢组和FGM浓度的变化。我们的结果表明，尽管粪便代谢组随压力变化（p <0.001），但FGM的平均浓度却没有变化（Levene检验：F比率：0.87，p值：0.44）。此外，我们表明，在应激反应中观察到的动物间变异与个体粪便微生物群相关。该结果与最近的发现一致，表明肠道微生物组是应激反应的关键介质。我们得出的结论是，基于NMR的粪便代谢组学被证明是评估压力反应的可靠方法，并且其未来在范围动物的压力监测研究中的应用可能比FGM分析更合适。

更新日期：2020-01-09

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