Epithelial integrity and barrier function are maintained during cytokinesis in vertebrate epithelial tissues. The changes in localization and the roles of tricellular tight junction molecule lipolysis-stimulated lipoprotein receptor (LSR) during cytokinesis are not well known, although new tricellular tight junctions form at the flank of the midbody during cytokinesis. In this study, we investigated the changes in localization and the role of LSR at the midbody and centrosome during cytokinesis using human endometrial carcinoma cell line Sawano, comparing the tricellular tight junction molecule tricellulin; bicellular tight junction molecules occludin, claudin-7, zonula occludens-1, and cingulin; and the epithelial polarized related molecules apoptosis-stimulating of p53 protein 2, PAR3, and yes-associated protein. During cytokinesis induced by treatment with taxol, the epithelial barrier was maintained and the tricellular tight junction molecules LSR and tricellulin were concentrated at the flank of the acetylated tubulin-positive midbody and in γ-tubulin-positive centrosomes with the dynein adaptor Hook2, whereas the other molecules were localized there as well. All the molecules disappeared by knockdown using small interfering RNAs. Furthermore, by the knockdown of Hook2, the epithelial barrier was maintained and most of the molecules disappeared from the centrosome. These findings suggest that LSR may play crucial roles not only in barrier function but also in cytokinesis.

中文翻译：

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在人类上皮细胞胞质分裂过程中，三细胞紧密连接分子LSR在中体和中心体的定位。

在脊椎动物上皮组织的胞质分裂过程中维持上皮的完整性和屏障功能。尽管胞质分裂过程中中体侧翼形成了新的三细胞紧密联接，但是在胞质分裂过程中三细胞紧密连接分子脂解刺激的脂蛋白受体（LSR）的定位变化和作用并不为人所知。在这项研究中，我们使用人子宫内膜癌细胞系Sawano调查了胞质分裂过程中LSR在中体和中心体中的定位及其作用的变化，比较了三细胞紧密连接分子tricellulin。双细胞紧密连接分子occludin，claudin-7，zonula occludens-1和cingulin；以及上皮极化相关分子对p53蛋白2，PAR3和yes相关蛋白的凋亡的刺激作用。在紫杉醇治疗诱导的胞质分裂过程中，上皮屏障得以维持，三细胞紧密连接分子LSR和三纤维蛋白通过动力蛋白适配器Hook2集中在乙酰化微管蛋白阳性中体的侧面和γ-微管蛋白阳性中心体的侧面。其他分子也位于那里。所有分子均通过使用小分子干扰RNA敲低而消失。此外，通过敲除Hook2，上皮屏障得以维持，大多数分子从中心体中消失。这些发现表明，LSR可能不仅在屏障功能中而且在胞质分裂中起关键作用。维持上皮屏障，三细胞紧密连接分子LSR和三纤维蛋白集中在乙酰化微管蛋白阳性中体的侧腹和带有动力蛋白衔接子Hook2的γ-微管蛋白阳性中心体中，而其他分子也位于那里。所有分子均通过使用小分子干扰RNA敲低而消失。此外，通过敲除Hook2，上皮屏障得以维持，大多数分子从中心体中消失。这些发现表明，LSR不仅在屏障功能中而且在胞质分裂中都可能起关键作用。维持上皮屏障，三细胞紧密连接分子LSR和三纤维蛋白集中在乙酰化微管蛋白阳性中体的侧腹和带有动力蛋白衔接子Hook2的γ-微管蛋白阳性中心体中，而其他分子也位于那里。所有分子均通过使用小分子干扰RNA敲低而消失。此外，通过敲除Hook2，上皮屏障得以维持，大多数分子从中心体中消失。这些发现表明，LSR可能不仅在屏障功能中而且在胞质分裂中起关键作用。通过Hook2的敲低，上皮屏障得以维持，大多数分子从中心体中消失。这些发现表明，LSR可能不仅在屏障功能中而且在胞质分裂中起关键作用。通过Hook2的敲低，上皮屏障得以维持，大多数分子从中心体中消失。这些发现表明，LSR可能不仅在屏障功能中而且在胞质分裂中起关键作用。